Detailed information of TA system TA204083

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 226229..226486
• Replicon: chromosome
• Accession: NZ_CP075649
• Organism: Escherichia coli strain STEC17-190

Toxin (Protein)

• Gene name: hokA
• Uniprot ID: E9T8R9
• Locus tag: JFX56_RS01045
• Protein ID: WP_001135746.1
• Coordinates: 226334..226486 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokA
Locus tag	-
Coordinates	226229..226283 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX56_RS01025	221454..222449	-	996	WP_001182653.1	O-acetyltransferase WecH	-
JFX56_RS01030	222624..222923	+	300	WP_000980111.1	YsaB family lipoprotein	-
JFX56_RS01035	223018..223929	+	912	WP_001168544.1	glycine--tRNA ligase subunit alpha	-
JFX56_RS01040	223939..226008	+	2070	WP_001291771.1	glycine--tRNA ligase subunit beta	-
-	226229..226283	-	55	-	-	Antitoxin
JFX56_RS01045	226334..226486	+	153	WP_001135746.1	type I toxin-antitoxin system toxin HokA	Toxin
JFX56_RS01050	226475..226534	-	60	WP_212591412.1	hypothetical protein	-
JFX56_RS01055	226674..226886	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
JFX56_RS01060	227167..227457	-	291	WP_000455798.1	HTH-type transcriptional regulator	-
JFX56_RS01065	227891..228601	+	711	WP_000190517.1	DUF3053 domain-containing protein	-
JFX56_RS01070	228651..229625	-	975	WP_000805027.1	glyoxylate/hydroxypyruvate reductase GhrB	-
JFX56_RS01075	229729..230388	-	660	WP_000747625.1	OmpA family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (5-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5928.14 Da
• Isoelectric Point: 7.7146

>T204083 WP_001135746.1 NZ_CP075649:226334-226486 [Escherichia coli]
MPQKYRLLSLIVICYTLLFFTWMIRDSLCELHIKQGSYELAAFLACNLKE

Nucleotide

• Length: 153 bp

>T204083 NZ_CP100530:c2147273-2146899 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGTTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCGGATGAACGCGTGATTGAGCTGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 55 bp

>AT204083 NZ_CP075649:c226283-226229 [Escherichia coli]
GTTCAGGCATAGGGTAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Structures

Toxin

• AlphaFold DB: A0A0H0JN89

Antitoxin