Detailed information of TA system TA204076

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108358..108778
• Replicon: plasmid pS19_141-a
• Accession: NZ_CP075641
• Organism: Escherichia coli strain S19-141

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JFX59_RS24975
• Protein ID: WP_223200913.1
• Coordinates: 108358..108480 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	108584..108778 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX59_RS24945 (104170)	104170..104553	-	384	WP_001585112.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JFX59_RS24950 (104886)	104886..105476	+	591	WP_279600944.1	transglycosylase SLT domain-containing protein	-
JFX59_RS24955 (105772)	105772..106593	-	822	WP_024213651.1	DUF932 domain-containing protein	-
JFX59_RS24960 (106712)	106712..106999	-	288	WP_000107542.1	hypothetical protein	-
JFX59_RS24965 (107155)	107155..107457	-	303	WP_024213649.1	hypothetical protein	-
JFX59_RS24970 (107760)	107760..108057	+	298	Protein_106	hypothetical protein	-
JFX59_RS24975 (108358)	108358..108480	-	123	WP_223200913.1	Hok/Gef family protein	Toxin
JFX59_RS25930 (108425)	108425..108577	-	153	Protein_108	DUF5431 family protein	-
- (108584)	108584..108778	-	195	NuclAT_0	-	Antitoxin
- (108584)	108584..108778	-	195	NuclAT_0	-	Antitoxin
- (108584)	108584..108778	-	195	NuclAT_0	-	Antitoxin
- (108584)	108584..108778	-	195	NuclAT_0	-	Antitoxin
JFX59_RS24980 (108727)	108727..109041	-	315	Protein_109	theronine dehydrogenase	-
JFX59_RS24985 (109038)	109038..109757	-	720	WP_001276237.1	plasmid SOS inhibition protein A	-
JFX59_RS24990 (109754)	109754..110188	-	435	WP_024213646.1	conjugation system SOS inhibitor PsiB	-
JFX59_RS24995 (110243)	110243..112201	-	1959	WP_024213645.1	ParB/RepB/Spo0J family partition protein	-
JFX59_RS25000 (112267)	112267..112500	-	234	WP_021564200.1	DUF905 family protein	-
JFX59_RS25005 (112558)	112558..113091	-	534	WP_024213643.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..133041	133041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T204076 WP_223200913.1 NZ_CP075641:c108480-108358 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T204076 NZ_CP100530:731388-731684 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Nucleotide

• Length: 195 bp

>AT204076 NZ_CP075641:c108778-108584 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCTGGTATATGGGCT
ATGATGTGCCCGGAGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin