Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 65860..66113 Replicon plasmid pS19_141-a
Accession NZ_CP075641
Organism Escherichia coli strain S19-141

Toxin (Protein)


Gene name srnB Uniprot ID A0A148HBD8
Locus tag JFX59_RS24720 Protein ID WP_001336447.1
Coordinates 65860..66009 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 66057..66113 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX59_RS24695 (62945) 62945..63184 - 240 WP_000859017.1 winged helix-turn-helix transcriptional regulator -
JFX59_RS24705 (64160) 64160..65017 - 858 WP_000130991.1 incFII family plasmid replication initiator RepA -
JFX59_RS24710 (65010) 65010..65084 - 75 WP_001365705.1 RepA leader peptide Tap -
JFX59_RS24715 (65319) 65319..65576 - 258 WP_000084404.1 replication regulatory protein RepA -
JFX59_RS24720 (65860) 65860..66009 - 150 WP_001336447.1 Hok/Gef family protein Toxin
- (66057) 66057..66113 + 57 NuclAT_1 - Antitoxin
- (66057) 66057..66113 + 57 NuclAT_1 - Antitoxin
- (66057) 66057..66113 + 57 NuclAT_1 - Antitoxin
- (66057) 66057..66113 + 57 NuclAT_1 - Antitoxin
JFX59_RS26095 (66288) 66288..66422 - 135 WP_001058542.1 hypothetical protein -
JFX59_RS24730 (66773) 66773..67234 - 462 WP_000760084.1 thermonuclease family protein -
JFX59_RS24735 (67977) 67977..68180 - 204 WP_038811942.1 hypothetical protein -
JFX59_RS24740 (68330) 68330..68887 - 558 WP_024213676.1 fertility inhibition protein FinO -
JFX59_RS24745 (68923) 68923..69291 - 369 WP_000722534.1 NirD/YgiW/YdeI family stress tolerance protein -
JFX59_RS24750 (69374) 69374..70120 - 747 WP_024213675.1 type-F conjugative transfer system pilin acetylase TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..133041 133041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5572.69 Da        Isoelectric Point: 8.7678

>T204073 WP_001336447.1 NZ_CP075641:c66009-65860 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T204073 NZ_CP100530:205025-205132 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGTATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 57 bp

>AT204073 NZ_CP075641:66057-66113 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A148HBD8

Antitoxin

Download structure file

References