Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3868199..3868998 Replicon chromosome
Accession NZ_CP075640
Organism Escherichia coli strain S19-141

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag JFX59_RS18665 Protein ID WP_000347267.1
Coordinates 3868199..3868663 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JFX59_RS18670 Protein ID WP_001307405.1
Coordinates 3868663..3868998 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX59_RS18635 (3863200) 3863200..3863634 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JFX59_RS18640 (3863652) 3863652..3864530 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
JFX59_RS18645 (3864520) 3864520..3865299 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JFX59_RS18650 (3865310) 3865310..3865783 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JFX59_RS18655 (3865806) 3865806..3867086 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
JFX59_RS18660 (3867335) 3867335..3868144 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JFX59_RS18665 (3868199) 3868199..3868663 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JFX59_RS18670 (3868663) 3868663..3868998 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JFX59_RS18675 (3869147) 3869147..3870718 - 1572 WP_001273741.1 galactarate dehydratase -
JFX59_RS18680 (3871093) 3871093..3872427 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JFX59_RS18685 (3872443) 3872443..3873213 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T204069 WP_000347267.1 NZ_CP075640:c3868663-3868199 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T204069 NZ_CP100525:c4181516-4181343 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT204069 WP_001307405.1 NZ_CP075640:c3868998-3868663 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204069 NZ_CP100525:c4181840-4181544 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References