Detailed information of TA system TA204066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2992740..2993342
• Replicon: chromosome
• Accession: NZ_CP075640
• Organism: Escherichia coli strain S19-141

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JFX59_RS14435
• Protein ID: WP_000897305.1
• Coordinates: 2993031..2993342 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFX59_RS14430
• Protein ID: WP_000356397.1
• Coordinates: 2992740..2993030 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX59_RS14405 (2988684)	2988684..2989586	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JFX59_RS14410 (2989583)	2989583..2990218	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JFX59_RS14415 (2990215)	2990215..2991144	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JFX59_RS14420 (2991474)	2991474..2991716	-	243	WP_001087409.1	protein YiiF	-
JFX59_RS14425 (2991936)	2991936..2992154	-	219	WP_001315930.1	CopG family transcriptional regulator	-
JFX59_RS14430 (2992740)	2992740..2993030	-	291	WP_000356397.1	NadS family protein	Antitoxin
JFX59_RS14435 (2993031)	2993031..2993342	-	312	WP_000897305.1	hypothetical protein	Toxin
JFX59_RS14440 (2993571)	2993571..2994479	+	909	WP_001162704.1	alpha/beta hydrolase	-
JFX59_RS14445 (2994543)	2994543..2995484	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JFX59_RS14450 (2995529)	2995529..2995966	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JFX59_RS14455 (2995963)	2995963..2996835	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JFX59_RS14460 (2996829)	2996829..2997428	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204066 WP_000897305.1 NZ_CP075640:c2993342-2993031 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204066 NZ_CP100525:c3677550-3677152 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204066 WP_000356397.1 NZ_CP075640:c2993030-2992740 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204066 NZ_CP100525:c3677846-3677553 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7