Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2000142..2000683 Replicon chromosome
Accession NZ_CP075640
Organism Escherichia coli strain S19-141

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JFX59_RS09805 Protein ID WP_000615976.1
Coordinates 2000405..2000683 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JFX59_RS09800 Protein ID WP_000729704.1
Coordinates 2000142..2000402 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX59_RS09775 (1995925) 1995925..1996710 - 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -
JFX59_RS09780 (1996682) 1996682..1998394 + 1713 Protein_1931 flagellar biosynthesis protein FlhA -
JFX59_RS09785 (1998499) 1998499..1998777 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JFX59_RS09790 (1998770) 1998770..1999126 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JFX59_RS09795 (1999183) 1999183..1999956 - 774 WP_000543896.1 C40 family peptidase -
JFX59_RS09800 (2000142) 2000142..2000402 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JFX59_RS09805 (2000405) 2000405..2000683 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JFX59_RS09810 (2000839) 2000839..2001579 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JFX59_RS09815 (2001550) 2001550..2002317 - 768 WP_000333380.1 class II glutamine amidotransferase -
JFX59_RS09820 (2002523) 2002523..2003101 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T204062 WP_000615976.1 NZ_CP075640:2000405-2000683 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T204062 NZ_CP100525:2587686-2587793 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT204062 WP_000729704.1 NZ_CP075640:2000142-2000402 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT204062 NZ_CP100525:c2587639-2587573 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References