Detailed information of TA system TA204049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 103423..103925
• Replicon: chromosome
• Accession: NZ_CP075640
• Organism: Escherichia coli strain S19-141

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: JFX59_RS00505
• Protein ID: WP_000767829.1
• Coordinates: 103671..103925 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: JFX59_RS00500
• Protein ID: WP_001259255.1
• Coordinates: 103423..103674 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX59_RS00475 (98601)	98601..99668	-	1068	WP_000080131.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
JFX59_RS00480 (99668)	99668..100738	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
JFX59_RS00485 (100735)	100735..102039	-	1305	WP_001368033.1	histidinol dehydrogenase	-
JFX59_RS00490 (102045)	102045..102944	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
JFX59_RS00495 (103090)	103090..103140	-	51	WP_001364200.1	his operon leader peptide	-
JFX59_RS00500 (103423)	103423..103674	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
JFX59_RS00505 (103671)	103671..103925	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
JFX59_RS00510 (104008)	104008..104832	+	825	WP_000754737.1	SDR family oxidoreductase	-
JFX59_RS00515 (104878)	104878..105807	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
JFX59_RS00520 (106022)	106022..106084	+	63	WP_010723108.1	membrane protein YoeI	-
JFX59_RS00525 (106074)	106074..107432	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
JFX59_RS00530 (107611)	107611..108669	+	1059	WP_000492340.1	thiosulfate utilization transporter TsuA/YeeE	-
JFX59_RS00535 (108683)	108683..108910	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T204049 WP_000767829.1 NZ_CP075640:103671-103925 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T204049 NZ_CP100520:c5122442-5122059 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCTTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT204049 WP_001259255.1 NZ_CP075640:103423-103674 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT204049 NZ_CP100520:c5122684-5122442 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0