Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 119513..119767 Replicon plasmid pSTEC2018_166-a
Accession NZ_CP075633
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag JFX80_RS26315 Protein ID WP_001312851.1
Coordinates 119618..119767 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 119513..119569 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS26285 (114967) 114967..115713 + 747 WP_000205749.1 conjugal transfer pilus acetylase TraX -
JFX80_RS26290 (115772) 115772..116632 + 861 WP_000704512.1 alpha/beta hydrolase -
JFX80_RS26295 (116735) 116735..117292 + 558 WP_000139316.1 fertility inhibition protein FinO -
JFX80_RS26300 (117446) 117446..117649 + 204 WP_001336517.1 hypothetical protein -
JFX80_RS26305 (118391) 118391..118852 + 462 WP_000760081.1 thermonuclease family protein -
JFX80_RS26310 (118940) 118940..119338 + 399 WP_000675594.1 hypothetical protein -
- (119513) 119513..119569 - 57 NuclAT_0 - Antitoxin
- (119513) 119513..119569 - 57 NuclAT_0 - Antitoxin
- (119513) 119513..119569 - 57 NuclAT_0 - Antitoxin
- (119513) 119513..119569 - 57 NuclAT_0 - Antitoxin
JFX80_RS26315 (119618) 119618..119767 + 150 WP_001312851.1 Hok/Gef family protein Toxin
JFX80_RS26320 (120051) 120051..120311 + 261 WP_000083819.1 replication regulatory protein RepA -
JFX80_RS27480 (120404) 120404..120589 + 186 Protein_125 plasmid copy control protein CopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD senB / hlyC / hlyA / hlyB / hlyD / iutA / iucD / iucC / iucB / iucA 1..120601 120601


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T204043 WP_001312851.1 NZ_CP075633:119618-119767 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T204043 NZ_CP100520:1890308-1890410 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 57 bp

>AT204043 NZ_CP075633:c119569-119513 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References