Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4148981..4149780 Replicon chromosome
Accession NZ_CP075632
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag JFX80_RS20455 Protein ID WP_000347267.1
Coordinates 4148981..4149445 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JFX80_RS20460 Protein ID WP_001307405.1
Coordinates 4149445..4149780 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS20425 (4143982) 4143982..4144416 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JFX80_RS20430 (4144434) 4144434..4145312 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
JFX80_RS20435 (4145302) 4145302..4146081 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JFX80_RS20440 (4146092) 4146092..4146565 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JFX80_RS20445 (4146588) 4146588..4147868 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
JFX80_RS20450 (4148117) 4148117..4148926 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JFX80_RS20455 (4148981) 4148981..4149445 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JFX80_RS20460 (4149445) 4149445..4149780 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JFX80_RS20465 (4149929) 4149929..4151500 - 1572 WP_001273741.1 galactarate dehydratase -
JFX80_RS20475 (4152513) 4152513..4153847 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JFX80_RS20480 (4153863) 4153863..4154633 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T204033 WP_000347267.1 NZ_CP075632:c4149445-4148981 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T204033 NZ_CP100515:c1261387-1261169 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT204033 WP_001307405.1 NZ_CP075632:c4149780-4149445 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204033 NZ_CP100515:c1261788-1261414 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References