Detailed information of TA system TA204030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3282567..3283169
• Replicon: chromosome
• Accession: NZ_CP075632
• Organism: Escherichia coli strain 2018-166

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JFX80_RS16210
• Protein ID: WP_000897305.1
• Coordinates: 3282858..3283169 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFX80_RS16205
• Protein ID: WP_000356397.1
• Coordinates: 3282567..3282857 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX80_RS16180 (3278511)	3278511..3279413	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JFX80_RS16185 (3279410)	3279410..3280045	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JFX80_RS16190 (3280042)	3280042..3280971	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JFX80_RS16195 (3281301)	3281301..3281543	-	243	WP_001087409.1	protein YiiF	-
JFX80_RS16200 (3281763)	3281763..3281981	-	219	WP_001315930.1	CopG family transcriptional regulator	-
JFX80_RS16205 (3282567)	3282567..3282857	-	291	WP_000356397.1	NadS family protein	Antitoxin
JFX80_RS16210 (3282858)	3282858..3283169	-	312	WP_000897305.1	hypothetical protein	Toxin
JFX80_RS16215 (3283398)	3283398..3284306	+	909	WP_001162704.1	alpha/beta hydrolase	-
JFX80_RS16220 (3284370)	3284370..3285311	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JFX80_RS16225 (3285356)	3285356..3285793	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JFX80_RS16230 (3285790)	3285790..3286662	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JFX80_RS16235 (3286656)	3286656..3287255	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204030 WP_000897305.1 NZ_CP075632:c3283169-3282858 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204030 NZ_CP100515:520329-520613 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204030 WP_000356397.1 NZ_CP075632:c3282857-3282567 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204030 NZ_CP100515:520097-520339 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGTGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7