Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2301509..2302050 Replicon chromosome
Accession NZ_CP075632
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JFX80_RS11515 Protein ID WP_000615976.1
Coordinates 2301772..2302050 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JFX80_RS11510 Protein ID WP_000729704.1
Coordinates 2301509..2301769 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS11485 (2297292) 2297292..2298077 - 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -
JFX80_RS11490 (2298049) 2298049..2299761 + 1713 Protein_2266 flagellar biosynthesis protein FlhA -
JFX80_RS11495 (2299866) 2299866..2300144 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JFX80_RS11500 (2300137) 2300137..2300493 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JFX80_RS11505 (2300550) 2300550..2301323 - 774 WP_000543896.1 C40 family peptidase -
JFX80_RS11510 (2301509) 2301509..2301769 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JFX80_RS11515 (2301772) 2301772..2302050 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JFX80_RS11520 (2302206) 2302206..2302946 + 741 WP_001225685.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JFX80_RS11525 (2302917) 2302917..2303684 - 768 WP_000333380.1 class II glutamine amidotransferase -
JFX80_RS11530 (2303890) 2303890..2304468 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T204026 WP_000615976.1 NZ_CP075632:2301772-2302050 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T204026 NZ_CP100511:c5280338-5279955 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT204026 WP_000729704.1 NZ_CP075632:2301509-2301769 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT204026 NZ_CP100511:c5280580-5280338 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATACCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References