Detailed information of TA system TA204003

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41151..41405
• Replicon: plasmid pP15-385-a
• Accession: NZ_CP075628
• Organism: Escherichia coli strain P15-385

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A1D7QA06
• Locus tag: JFX93_RS24295
• Protein ID: WP_001367749.1
• Coordinates: 41151..41300 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	41344..41405 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX93_RS24265 (36764)	36764..37186	-	423	WP_001212724.1	Exc2 family lipoprotein	-
JFX93_RS24270 (37469)	37469..37906	+	438	Protein_38	transposase	-
JFX93_RS24275 (38237)	38237..38476	-	240	WP_000859018.1	winged helix-turn-helix transcriptional regulator	-
JFX93_RS24280 (39449)	39449..40306	-	858	WP_000130938.1	incFII family plasmid replication initiator RepA	-
JFX93_RS24285 (40299)	40299..40373	-	75	WP_001367777.1	RepA leader peptide Tap	-
JFX93_RS24290 (40619)	40619..40867	-	249	WP_000083842.1	replication regulatory protein RepA	-
JFX93_RS24295 (41151)	41151..41300	-	150	WP_001367749.1	Hok/Gef family protein	Toxin
- (41344)	41344..41405	+	62	NuclAT_0	-	Antitoxin
- (41344)	41344..41405	+	62	NuclAT_0	-	Antitoxin
- (41344)	41344..41405	+	62	NuclAT_0	-	Antitoxin
- (41344)	41344..41405	+	62	NuclAT_0	-	Antitoxin
JFX93_RS24300 (41580)	41580..41963	-	384	WP_001109066.1	hypothetical protein	-
JFX93_RS24305 (42066)	42066..42527	-	462	WP_000417388.1	thermonuclease family protein	-
JFX93_RS24310 (43272)	43272..43475	-	204	WP_001327131.1	hypothetical protein	-
JFX93_RS24315 (43629)	43629..44192	-	564	WP_000139382.1	fertility inhibition protein FinO	-
JFX93_RS24320 (44295)	44295..45155	-	861	WP_000868985.1	alpha/beta hydrolase	-
JFX93_RS24325 (45214)	45214..45954	-	741	WP_198793062.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC	1..118360	118360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5528.64 Da
• Isoelectric Point: 8.7678

>T204003 WP_001367749.1 NZ_CP075628:c41300-41151 [Escherichia coli]
MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T204003 NZ_CP100500:c4869097-4868747 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT204003 NZ_CP075628:41344-41405 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A1D7QA06

Antitoxin