Detailed information of TA system TA204002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4934868..4935470
• Replicon: chromosome
• Accession: NZ_CP075627
• Organism: Escherichia coli strain P15-385

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JFX93_RS23920
• Protein ID: WP_000897305.1
• Coordinates: 4934868..4935179 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFX93_RS23925
• Protein ID: WP_000356397.1
• Coordinates: 4935180..4935470 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX93_RS23895 (4930782)	4930782..4931381	+	600	WP_001295269.1	glucose-1-phosphatase	-
JFX93_RS23900 (4931375)	4931375..4932247	+	873	WP_000920762.1	virulence factor BrkB family protein	-
JFX93_RS23905 (4932244)	4932244..4932681	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JFX93_RS23910 (4932726)	4932726..4933667	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JFX93_RS23915 (4933731)	4933731..4934639	-	909	WP_001367979.1	alpha/beta hydrolase	-
JFX93_RS23920 (4934868)	4934868..4935179	+	312	WP_000897305.1	hypothetical protein	Toxin
JFX93_RS23925 (4935180)	4935180..4935470	+	291	WP_000356397.1	NadS family protein	Antitoxin
JFX93_RS23930 (4936056)	4936056..4936274	+	219	WP_001295676.1	CopG family transcriptional regulator	-
JFX93_RS23935 (4936493)	4936493..4936735	+	243	WP_001087409.1	protein YiiF	-
JFX93_RS23940 (4937065)	4937065..4937994	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JFX93_RS23945 (4937991)	4937991..4938626	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JFX93_RS23950 (4938623)	4938623..4939525	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T204002 WP_000897305.1 NZ_CP075627:4934868-4935179 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T204002 NZ_CP100500:c4733255-4732968 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT204002 WP_000356397.1 NZ_CP075627:4935180-4935470 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT204002 NZ_CP100500:c4732981-4732679 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7