Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4137932..4138731 Replicon chromosome
Accession NZ_CP075627
Organism Escherichia coli strain P15-385

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag JFX93_RS20060 Protein ID WP_000347267.1
Coordinates 4138267..4138731 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JFX93_RS20055 Protein ID WP_001307405.1
Coordinates 4137932..4138267 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX93_RS20040 (4133717) 4133717..4134487 - 771 WP_001058232.1 2-dehydro-3-deoxyglucarate aldolase -
JFX93_RS20045 (4134503) 4134503..4135837 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JFX93_RS20050 (4136212) 4136212..4137783 + 1572 WP_001273741.1 galactarate dehydratase -
JFX93_RS20055 (4137932) 4137932..4138267 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JFX93_RS20060 (4138267) 4138267..4138731 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JFX93_RS20065 (4138786) 4138786..4139595 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JFX93_RS20070 (4139844) 4139844..4141124 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
JFX93_RS20075 (4141147) 4141147..4141620 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JFX93_RS20080 (4141631) 4141631..4142410 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JFX93_RS20085 (4142400) 4142400..4143278 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
JFX93_RS20090 (4143296) 4143296..4143730 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4127276..4138731 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T204000 WP_000347267.1 NZ_CP075627:4138267-4138731 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T204000 NZ_CP100500:4396604-4396882 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATATTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT204000 WP_001307405.1 NZ_CP075627:4137932-4138267 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204000 NZ_CP100500:4396341-4396601 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References