Detailed information of TA system TA203915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5523227..5523882
• Replicon: chromosome
• Accession: NZ_CP075567
• Organism: Pseudomonas fitomaticsae strain FIT81

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KJY40_RS24935
• Protein ID: WP_065261338.1
• Coordinates: 5523538..5523882 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KJY40_RS24930
• Protein ID: WP_007956715.1
• Coordinates: 5523227..5523541 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJY40_RS24905 (KJY40_24855)	5518367..5519848	+	1482	WP_230733382.1	MBOAT family protein	-
KJY40_RS24910 (KJY40_24860)	5519859..5520986	+	1128	WP_230733384.1	hypothetical protein	-
KJY40_RS24915 (KJY40_24865)	5521065..5521589	+	525	WP_230733386.1	hypothetical protein	-
KJY40_RS24920 (KJY40_24870)	5521763..5522467	+	705	WP_039765656.1	hypothetical protein	-
KJY40_RS24925 (KJY40_24875)	5522537..5523028	-	492	WP_007956714.1	RidA family protein	-
KJY40_RS24930 (KJY40_24880)	5523227..5523541	-	315	WP_007956715.1	helix-turn-helix domain-containing protein	Antitoxin
KJY40_RS24935 (KJY40_24885)	5523538..5523882	-	345	WP_065261338.1	toxin	Toxin
KJY40_RS24940 (KJY40_24890)	5524008..5524895	-	888	WP_085668163.1	heme o synthase	-
KJY40_RS24945 (KJY40_24895)	5524906..5525241	-	336	WP_007956718.1	cytochrome o ubiquinol oxidase subunit IV	-
KJY40_RS24950 (KJY40_24900)	5525241..5525867	-	627	WP_007956720.1	cytochrome o ubiquinol oxidase subunit III	-
KJY40_RS24955 (KJY40_24905)	5525871..5527901	-	2031	WP_127799800.1	cytochrome o ubiquinol oxidase subunit I	-
KJY40_RS24960 (KJY40_24910)	5527905..5528846	-	942	WP_011335843.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13444.31 Da
• Isoelectric Point: 9.2890

>T203915 WP_065261338.1 NZ_CP075567:c5523882-5523538 [Pseudomonas fitomaticsae]
MRTVFFETTLFTANLGHYLTDDEYRELQTYMQSNPHAGDVMPRTGGFRKLRWVDVRRGKGRRGGLRVIYYWLMNDGQFWM
FAIYDKDELENLTAEQEKALKGAIEKELKTRGTP

Nucleotide

• Length: 345 bp

>T203915 NZ_CP100478:c4534775-4534242 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCCAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11859.77 Da
• Isoelectric Point: 10.3048

>AT203915 WP_007956715.1 NZ_CP075567:c5523541-5523227 [Pseudomonas fitomaticsae]
MKKRDLFAELMQGVDEMAAQREGKITLRNTTLEDKPAPEVGAQEIVALREKLHMSQAVFARRIRTSPSTLRNWEQEKSKP
NAQAALLIRLVEKFPDMVERLSAV

Nucleotide

• Length: 315 bp

>AT203915 NZ_CP100478:c4535052-4534786 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA