Detailed information of TA system TA203910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 525378..525982
• Replicon: chromosome
• Accession: NZ_CP075567
• Organism: Pseudomonas fitomaticsae strain FIT81

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3KJP2
• Locus tag: KJY40_RS02325
• Protein ID: WP_011331970.1
• Coordinates: 525668..525982 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KJY40_RS02320
• Protein ID: WP_098966690.1
• Coordinates: 525378..525665 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJY40_RS02295 (KJY40_02295)	520607..521053	-	447	WP_170844915.1	ATP-dependent zinc protease	-
KJY40_RS02300 (KJY40_02300)	521305..521712	+	408	WP_230734732.1	S4 domain-containing protein	-
KJY40_RS02305 (KJY40_02305)	521782..522582	-	801	WP_230734734.1	phosphatase PAP2 family protein	-
KJY40_RS02310 (KJY40_02310)	522687..523589	+	903	WP_085608616.1	Hsp33 family molecular chaperone HslO	-
KJY40_RS02315 (KJY40_02315)	523771..525312	+	1542	WP_007952682.1	phosphoenolpyruvate carboxykinase	-
KJY40_RS02320 (KJY40_02320)	525378..525665	-	288	WP_098966690.1	NadS family protein	Antitoxin
KJY40_RS02325 (KJY40_02325)	525668..525982	-	315	WP_011331970.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KJY40_RS02330 (KJY40_02330)	526094..526393	-	300	WP_230734736.1	hypothetical protein	-
KJY40_RS02335 (KJY40_02335)	526944..528071	-	1128	WP_230734738.1	hypothetical protein	-
KJY40_RS02340 (KJY40_02340)	528174..528455	-	282	WP_230734739.1	hypothetical protein	-
KJY40_RS02345 (KJY40_02345)	528760..529263	+	504	WP_230734741.1	polysaccharide deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11847.76 Da
• Isoelectric Point: 10.3534

>T203910 WP_011331970.1 NZ_CP075567:c525982-525668 [Pseudomonas fitomaticsae]
MIFIETRIFTRRVKELLDDDTYAAFQKQLVVSPSIGDVIEGTGGIRKTRIAAKGHGKRGGARVIYYHFVSASQIGLLMIY
PKNEQHDLSSDERKALKVLIEKWR

Nucleotide

• Length: 315 bp

>T203910 NZ_CP100478:734001-734486 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGAATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTAGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10777.34 Da
• Isoelectric Point: 6.8814

>AT203910 WP_098966690.1 NZ_CP075567:c525665-525378 [Pseudomonas fitomaticsae]
MSKFFDELMESVQEMDEIVRGERTPSREFHVDALQVKEIRKATGLTQTKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAH

Nucleotide

• Length: 288 bp

>AT203910 NZ_CP100478:733711-734004 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: Q3KJP2