Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 358539..359185 Replicon chromosome
Accession NZ_CP075552
Organism Klebsiella pneumoniae strain RGF99-1

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag KI681_RS01655 Protein ID WP_004174016.1
Coordinates 358539..358886 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4S7L580
Locus tag KI681_RS01660 Protein ID WP_019725405.1
Coordinates 358886..359185 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI681_RS01645 (KI681_01645) 354465..355898 + 1434 WP_002920564.1 glycogen synthase GlgA -
KI681_RS01650 (KI681_01650) 355916..358363 + 2448 WP_121593178.1 glycogen phosphorylase -
KI681_RS01655 (KI681_01655) 358539..358886 + 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KI681_RS01660 (KI681_01660) 358886..359185 + 300 WP_019725405.1 XRE family transcriptional regulator Antitoxin
KI681_RS01665 (KI681_01665) 359248..360756 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
KI681_RS01670 (KI681_01670) 360961..361290 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
KI681_RS01675 (KI681_01675) 361341..362171 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
KI681_RS01680 (KI681_01680) 362221..362979 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T203850 WP_004174016.1 NZ_CP075552:358539-358886 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T203850 NZ_CP100397:5560506-5560853 [Streptomyces rimosus subsp. rimosus]
ATGCGGCGCGGTGACCTCTATCTGGTTGATCTGGAGCCGGTCCGGGGGAGCGAGGCCAACAAGTACCGTCCTGCGGTGGT
CGTGTCCAACGACGCGGCCAATCGGTCCGTGCAGCGCGCCGGAAGGGGTGTGGTGACGGTCGTGCCTGTGACGGCCAACG
TCGCCCGGGTCTACCCGTTCCAGGTGCTGCTGACCGCCGACGACTGCGGGCTGCCGCGCGACTCGAAGGCCCAGTGCGAG
CAGGTGCGCGCGGTGTCCGTCGAGCGGCTGGGGCGGCGGGTCGGCAGCGTCCCGGCGCGCGTGATGGTTGGGCTGGACGC
GGCCCTGCGCAGGCATCTGGCGCTCTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10775.53 Da        Isoelectric Point: 9.7681

>AT203850 WP_019725405.1 NZ_CP075552:358886-359185 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT203850 NZ_CP100397:5560283-5560519 [Streptomyces rimosus subsp. rimosus]
ATGAAGATCAGCGTCAGCCTGCCGGAAGAGGACATCGCCTTCCTCGACGCGTACGGCAAGAAGACGGACGCCGATTCGCG
GTCGGCGGTGATCCACGCGGCGGTCGAGCTGCTGCGTGCCTCCGAGCTGGAGGCGGATTACGCCTCGGCGTGGGAGGAGT
GGGACGAGAGCGACGACGCGCTGCTGTGGGACGGGGTCTCCGGGGACGGGATCGGAAGCGCCGATGCGGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A4S7L580

References