Detailed information of TA system TA203837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3887923..3888520
• Replicon: chromosome
• Accession: NZ_CP075548
• Organism: Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: KI675_RS19100
• Protein ID: WP_004893639.1
• Coordinates: 3888203..3888520 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KI675_RS19095
• Protein ID: WP_004142561.1
• Coordinates: 3887923..3888210 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI675_RS19065 (3884130)	3884130..3884378	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
KI675_RS19070 (3884396)	3884396..3884737	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KI675_RS19075 (3884768)	3884768..3885883	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
KI675_RS19080 (3886063)	3886063..3886644	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
KI675_RS19085 (3886644)	3886644..3887012	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
KI675_RS19090 (3887132)	3887132..3887785	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
KI675_RS19095 (3887923)	3887923..3888210	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KI675_RS19100 (3888203)	3888203..3888520	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI675_RS19105 (3888705)	3888705..3889748	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
KI675_RS19110 (3890418)	3890418..3891284	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
KI675_RS19115 (3891393)	3891393..3892820	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T203837 WP_004893639.1 NZ_CP075548:c3888520-3888203 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T203837 NZ_CP100390:1338987-1339298 [Alkalimarinus sp. A2M4]
ATGGCTCAAATAACTTGGACTGAGCCAGCTCTAGAAAATCTGAATGATATTGCTGAGTATATTGCAGTTAGCAATCCGTA
TGCTGCTAAACAGTTAGTAGAAAACGTTTTCAGTAAGGTTCAAAGACTTGAGCAATTTCCTGATTCAGGCAGAGTGCCTG
AAGAAATTTCTACCCTAAATTACCGAGAAGTGGTGGTAAATCCTTGTCGTGTTTTCTATAAGGTTGATAACGATTCAGTC
TATATCCTTCACGTGATGAGGCAAGAAAGAGATTTACGTAAGTTTTTACTATCTACCGAAAATGAAAACTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT203837 WP_004142561.1 NZ_CP075548:c3888210-3887923 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT203837 NZ_CP100390:1338742-1338999 [Alkalimarinus sp. A2M4]
ATGAGAACCGAAGTCGTTACGACGTTAAAGAGGCAAGCAACGAAGCTGCTTGCTGATCTACATGAGTCAAAAGAACCTAT
TCTCATAACTGAACATGGTCAACCATCTGCATATTTACTTGATGTTGCTGATTACGAGTTGATGGTAGACCGGATGAAGA
TTCTTGAGGGTATCGCTAAAGGCGAGCAGGCTATTCGAGATGGCCGAACCTTGGGTATGGAGCAAGCTAGAGAGAAAATG
AATAAATGGCTCAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1