Detailed information of TA system TA203818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3614566..3615260
• Replicon: chromosome
• Accession: NZ_CP075487
• Organism: Escherichia coli strain NT1F34

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IGI26_RS18015
• Protein ID: WP_001263493.1
• Coordinates: 3614566..3614964 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IGI26_RS18020
• Protein ID: WP_000554757.1
• Coordinates: 3614967..3615260 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3610226)	3610226..3610306	-	81	NuclAT_9	-	-
- (3610226)	3610226..3610306	-	81	NuclAT_9	-	-
- (3610226)	3610226..3610306	-	81	NuclAT_9	-	-
- (3610226)	3610226..3610306	-	81	NuclAT_9	-	-
IGI26_RS17985 (3609566)	3609566..3610810	-	1245	WP_000189541.1	esterase FrsA	-
IGI26_RS17990 (3610902)	3610902..3611360	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IGI26_RS17995 (3611621)	3611621..3613078	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
IGI26_RS18000 (3613135)	3613135..3613656	-	522	Protein_3419	peptide chain release factor H	-
IGI26_RS18005 (3613655)	3613655..3613858	-	204	Protein_3420	RtcB family protein	-
IGI26_RS18010 (3614104)	3614104..3614556	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IGI26_RS18015 (3614566)	3614566..3614964	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IGI26_RS18020 (3614967)	3614967..3615260	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IGI26_RS18025 (3615312)	3615312..3616367	-	1056	WP_001226164.1	DNA polymerase IV	-
IGI26_RS18030 (3616438)	3616438..3617223	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IGI26_RS18035 (3617195)	3617195..3618907	+	1713	Protein_3426	flagellar biosynthesis protein FlhA	-
IGI26_RS18040 (3619123)	3619123..3619619	-	497	Protein_3427	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T203818 WP_001263493.1 NZ_CP075487:c3614964-3614566 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T203818 NZ_CP100351:2880386-2880676 [Desulfomicrobium sp. ZS1]
ATGCCACGTCTGATCTGGACCGCGGGGGCCGTTGACGGACTCGGTAAAATCCACCGCTTTCTTGCCGAAAAGGATGCGCA
GGCCGCTGCGAAAGCCCTGGACGCCATCCGCAAGGGAGCAAACATCCTGCGATACTTTCCCCAGGCCGGACGCCCGACGG
AGGACCTGGAACCGGAACATCGCGAATTGCTCATCCCGTTCGGGGCTTCAGGCTACGCCGTTTTCTACGAAACCATCGAC
GACTGCATATGCATCCTCACCGTCAAACATCAGAAAGAAGCGGGATACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT203818 WP_000554757.1 NZ_CP075487:c3615260-3614967 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT203818 NZ_CP100351:2880105-2880398 [Desulfomicrobium sp. ZS1]
ATGCCCTCGCAAGGACCGATCAAGCCCACGAGCATCAAGCTTCCCCTGGAATTGCACGCACGCGTGCAGCACATTGCCAT
GGTCCGTAAGCGTTCGGTTCATGAAGTCATGATCAGGGCGATGGAGACCTATGTGGACAGGGAGGAGAAGCGGGAGGCCA
TCCGTCAGGAGTGTATCAAGGCCCACGACGAGTATATGCTGACCGGACTGCATGTCACAGGCGAAGAGGTAGACGCATGG
ATTGATCAACTCGTTGATGGCAACGAGACGGAAATGCCCGCATGCCACGTCTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6