Detailed information of TA system TA203800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 647513..648312
• Replicon: chromosome
• Accession: NZ_CP075487
• Organism: Escherichia coli strain NT1F34

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: IGI26_RS03770
• Protein ID: WP_000347267.1
• Coordinates: 647513..647977 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: IGI26_RS03775
• Protein ID: WP_001302819.1
• Coordinates: 647977..648312 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI26_RS03740 (642514)	642514..642948	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
IGI26_RS03745 (642966)	642966..643844	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
IGI26_RS03750 (643834)	643834..644613	-	780	Protein_610	PTS N-acetylgalactosamine transporter subunit IIC	-
IGI26_RS03755 (644624)	644624..645097	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IGI26_RS03760 (645120)	645120..646400	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IGI26_RS03765 (646649)	646649..647458	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
IGI26_RS03770 (647513)	647513..647977	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IGI26_RS03775 (647977)	647977..648312	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IGI26_RS03780 (648461)	648461..650032	-	1572	WP_057107993.1	galactarate dehydratase	-
IGI26_RS03785 (650407)	650407..651741	+	1335	WP_193115198.1	galactarate/glucarate/glycerate transporter GarP	-
IGI26_RS03790 (651757)	651757..652527	+	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T203800 WP_000347267.1 NZ_CP075487:c647977-647513 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T203800 NZ_CP100338:565419-565679 [Streptococcus suis]
ATGTATCATTTAGAGTATTCTAAAAAGGCTCAAAAGCAAATTAAGAAATTAGATAGGCAAATTCAGAGGTTGTTGTTTGC
TTGGATTGATAAACACTTAGAAGGGACTGATAATCCACGCGCCAATGGTAAAGGTTTGACAGGCAATCATGCTAACGAGT
GGCGTTATCGTATCGGAGATTACCGACTGATTTGCGATATTCAAGATGATAAGTTGGTTATTTTAGCACTTGAGTTTGGA
CATCGTAAAGATGTCTATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT203800 WP_001302819.1 NZ_CP075487:c648312-647977 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT203800 NZ_CP100338:565196-565417 [Streptococcus suis]
ATGAGTACAGTGACAATCAGATTAAATCAAGAAGAAGAAGTATTCTTTAAAAGCTATGCACAATTAACAGGTCAAAGTCT
TTCTAGCCTTTTTAAGAAAGCTTTGGAGCGTGATATTGAAGATGAGTATGATTTAAAAATCTATCATCAAGCTTATGATG
AGTACAAAGCAGATCCAGAAACAATTAGTCATGCTGATTTTAAGAAAGAGCTAGGATTATAG

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4