Detailed information of TA system TA203797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4472908..4473510
• Replicon: chromosome
• Accession: NZ_CP075484
• Organism: Escherichia coli strain NT1N25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IGI28_RS21795
• Protein ID: WP_000897305.1
• Coordinates: 4473199..4473510 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IGI28_RS21790
• Protein ID: WP_000356397.1
• Coordinates: 4472908..4473198 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI28_RS21765 (4468834)	4468834..4469736	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IGI28_RS21770 (4469733)	4469733..4470368	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IGI28_RS21775 (4470365)	4470365..4471294	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IGI28_RS21780 (4471624)	4471624..4471866	-	243	WP_001086388.1	protein YiiF	-
IGI28_RS21785 (4472085)	4472085..4472303	-	219	WP_001295676.1	CopG family transcriptional regulator	-
IGI28_RS21790 (4472908)	4472908..4473198	-	291	WP_000356397.1	NadS family protein	Antitoxin
IGI28_RS21795 (4473199)	4473199..4473510	-	312	WP_000897305.1	hypothetical protein	Toxin
IGI28_RS21800 (4473739)	4473739..4474647	+	909	WP_137446804.1	alpha/beta hydrolase	-
IGI28_RS21805 (4474711)	4474711..4475652	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IGI28_RS21810 (4475697)	4475697..4476134	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
IGI28_RS21815 (4476131)	4476131..4477003	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IGI28_RS21820 (4476997)	4476997..4477596	-	600	WP_001315111.1	glucose-1-phosphatase	-
IGI28_RS21825 (4477695)	4477695..4478480	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T203797 WP_000897305.1 NZ_CP075484:c4473510-4473199 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T203797 NZ_CP100336:1321372-1322217 [Streptococcus suis]
ATGAACAAAGCTAAATTAACAGCACTCTGTCATAAAATATCAAAGAATACTGGTTTAACCTTTAATTCCGTCATGACCTA
TTACTTCCTAGAAGTCATTTTGAAAAAATTAAGTCAAAGTTCTTATTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TTTCGAATGTCATCGGAGTTGAATCTCGTAGCACTGTTGATATTGACTTTTTATTCCATCAAATAACACTCTCAGAAGAA
ACTGTTAAACAGCAACTCAAGGAAATTTTAGCAGATTCCGAAGAAGGTATATCTTTTGTGATTCAATCAATCACAGCTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTCGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGTGATGTTGTAACTCCTCAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGACAATTTTCCA
ATCATTGCTTATACAATTGAAACAATTCTAGCTGAAAAACTTCAAACCATCTATTCACGTAACTTCTTGAACAGTAGAAG
CAAAGATTTTTACGATGTTTATATTCTTTCCAAACTGAAGAAAGAAGACATTGACTTCATTCAGTTGAGAAATGCCTGTC
AGAGAACATTTTCATATCGCGAAACAGAACTCGATTTTGTAAAGATTATTGAACTACTCGAGAGGTTCAAATCTGACCCT
ATTCAGAATAAACAATGGCAAAATTATTCAAAAAAATATAGCTATACAAAAGGGATTTCACTTGCAGATGTTCTTGATGA
GATGATTCGTCTCATAACAGTTCTCATTTCTATAAATTCTGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT203797 WP_000356397.1 NZ_CP075484:c4473198-4472908 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT203797 NZ_CP100336:1320785-1321375 [Streptococcus suis]
ATGTCAAAAAAAGAGATTCTACTCGATTTTATAGAAAAGAACAATGGAATTGTCACAAACAAAGATTGTAAAGCACTAGG
TATTCCAACAATATATCTGACAAGACTAGAGAAAGAAGGCATTATCTTCCGAGTTGAAAAAGGTATTTTCCTAACCCAAA
ACGGAGACTATGATGAATACTACTTCTTTCAATATCGTTTTCCTAAGGCTATTTTCTCTTATATTTCGGCACTGTATCTA
CAACAGTTTACAGATGAAATTCCACAATATTTTGATGTGACAGTTCCTAGAGGCTACCGCTTTAATACTCCTCCAGCTAA
TCTAAATATTCACTTTGTCTCTAAAGAATACAGCGAGCTGGGAATGACTACTGTACCTACTCCTTTGGGAAACAACGTAA
GGGTATATGATTTTGAACGTATTATTTGTGATTTTGTAATACATCGAGAAAAGATTGATTCCGAACTCTTTGTCAAGACA
CTTCAATCTTATGGGAACTATCCTAAAAAAAATCTAGCAAAACTCTATGAATATGCGACAAAAATGAATACTTTAGAAAA
AGTTAAACAAACTCTGGAGGTTCTAATATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7