Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3658609..3659150 Replicon chromosome
Accession NZ_CP075484
Organism Escherichia coli strain NT1N25

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag IGI28_RS18015 Protein ID WP_000615976.1
Coordinates 3658872..3659150 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IGI28_RS18010 Protein ID WP_000729704.1
Coordinates 3658609..3658869 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI28_RS17985 (3654392) 3654392..3655177 - 786 WP_000207590.1 putative lateral flagellar export/assembly protein LafU -
IGI28_RS17990 (3655149) 3655149..3656861 + 1713 Protein_3442 flagellar biosynthesis protein FlhA -
IGI28_RS17995 (3656966) 3656966..3657244 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
IGI28_RS18000 (3657237) 3657237..3657593 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
IGI28_RS18005 (3657650) 3657650..3658423 - 774 WP_000543895.1 C40 family peptidase -
IGI28_RS18010 (3658609) 3658609..3658869 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IGI28_RS18015 (3658872) 3658872..3659150 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IGI28_RS18020 (3659306) 3659306..3660046 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
IGI28_RS18025 (3660017) 3660017..3660784 - 768 WP_000333380.1 class II glutamine amidotransferase -
IGI28_RS18030 (3660990) 3660990..3661568 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3657237..3668762 11525


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T203794 WP_000615976.1 NZ_CP075484:3658872-3659150 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T203794 NZ_CP100336:563114-563374 [Streptococcus suis]
ATGTATCATTTAGAGTATTCTAAAAAGGCTCAAAAGCAAATTAAGAAATTAGATAGGCAAATTCAGAGGTTGTTGTTTGC
TTGGATTGATAAACACTTAGAAGGGACTGATAATCCACGCGCCAATGGTAAAGGTTTGACAGGCAATCATGCTAACGAGT
GGCGTTATCGTATCGGAGATTACCGACTGATTTGCGATATTCAAGATGATAAGTTGGTTATTTTAGCACTTGAGTTTGGA
CATCGTAAAGATGTCTATTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT203794 WP_000729704.1 NZ_CP075484:3658609-3658869 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT203794 NZ_CP100336:562891-563112 [Streptococcus suis]
ATGAGTACAGTGACAATCAGATTAAATCAAGAAGAAGAAGTATTCTTTAAAAGCTATGCACAATTAACAGGTCAAAGTCT
TTCTAGCCTTTTTAAGAAAGCTTTGGAGCGTGATATTGAAGATGAGTATGATTTAAAAATCTATCATCAAGCTTATGATG
AGTACAAAGCAGATCCAGAAACAATTAGTCATGCTGATTTTAAGAAAGAGCTAGGATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References