Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 595201..596000 Replicon chromosome
Accession NZ_CP075484
Organism Escherichia coli strain NT1N25

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag IGI28_RS03400 Protein ID WP_000347267.1
Coordinates 595201..595665 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IGI28_RS03405 Protein ID WP_001307405.1
Coordinates 595665..596000 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI28_RS03370 (590202) 590202..590636 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
IGI28_RS03375 (590654) 590654..591532 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
IGI28_RS03380 (591522) 591522..592301 - 780 WP_166488258.1 PTS N-acetylgalactosamine transporter subunit IIC -
IGI28_RS03385 (592312) 592312..592785 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
IGI28_RS03390 (592808) 592808..594088 - 1281 WP_000681947.1 tagatose-bisphosphate aldolase subunit KbaZ -
IGI28_RS03395 (594337) 594337..595146 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
IGI28_RS03400 (595201) 595201..595665 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IGI28_RS03405 (595665) 595665..596000 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IGI28_RS03410 (596149) 596149..597720 - 1572 WP_001273741.1 galactarate dehydratase -
IGI28_RS03415 (598095) 598095..599429 + 1335 Protein_575 galactarate/glucarate/glycerate transporter GarP -
IGI28_RS03420 (599445) 599445..600215 + 771 WP_166488256.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 595201..608267 13066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T203775 WP_000347267.1 NZ_CP075484:c595665-595201 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T203775 NZ_CP100328:c2077616-2077359 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACATTGAAGAGAAT
CAATAAGATTATCAAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT203775 WP_001307405.1 NZ_CP075484:c596000-595665 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT203775 NZ_CP100328:c2077878-2077618 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References