Detailed information of TA system TA203772

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75795..76048
• Replicon: plasmid pNT1N25-76kb
• Accession: NZ_CP075483
• Organism: Escherichia coli strain NT1N25

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: IGI28_RS00465
• Protein ID: WP_001312851.1
• Coordinates: 75899..76048 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	75795..75854 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI28_RS00440 (72522)	72522..73268	+	747	WP_193115341.1	conjugal transfer pilus acetylase TraX	-
IGI28_RS00445 (73323)	73323..73883	+	561	WP_000139328.1	fertility inhibition protein FinO	-
IGI28_RS00450 (74015)	74015..74215	+	201	WP_015059022.1	hypothetical protein	-
IGI28_RS00455 (74601)	74601..75200	+	600	WP_032083981.1	PIN domain-containing protein	-
IGI28_RS00460 (75262)	75262..75594	+	333	WP_152916585.1	hypothetical protein	-
- (75795)	75795..75854	-	60	NuclAT_1	-	Antitoxin
- (75795)	75795..75854	-	60	NuclAT_1	-	Antitoxin
- (75795)	75795..75854	-	60	NuclAT_1	-	Antitoxin
- (75795)	75795..75854	-	60	NuclAT_1	-	Antitoxin
IGI28_RS00465 (75899)	75899..76048	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
IGI28_RS00470 (76332)	76332..76580	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / blaTEM-1B / blaCTX-M-55	-	1..76891	76891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T203772 WP_001312851.1 NZ_CP075483:75899-76048 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T203772 NZ_CP100328:c1285482-1284715 [Streptococcus suis]
ATGAGACTGGAAGAATTTAGTGAGGCTGAGTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCTATCTTGCTTGGTGGACAAAGCGGAGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AGGAATTTCAAGGTAATATCATTATTATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCCTGCAAGAA
AAGTATGGCAAGGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAC
GCAAGGCTACCATTTGCTGATTGAGGGAACCTTGCGAACTACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCTA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGTACCAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACTCAGGCTAGAGCAACACCAAAAGAACACCACGATGGGATTGTTGAGAACTTGGTTGATAA
CTTGAGGGAGTTAGAAAGTGAAAATCTCTTTGACCAGATTCAAATTTATCAAAGAGATAGAACTTGTATCTATGATTCTG
AAACTGCTGAAGGTTCCGCAGCAGAAGTGCTACAAGAATGCCTATTTGGAAAATGGAGCAAGGTAGAGGAGGAGATGGTG
AAGGAGAGTGAACTCCTACTTAAGGAATTACTTGAGAAAAATAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT203772 NZ_CP075483:c75854-75795 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin