Detailed information of TA system TA203740

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32970..33239
• Replicon: plasmid pNT1W25-82k
• Accession: NZ_CP075478
• Organism: Escherichia coli strain NT1W25

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: IGI33_RS22575
• Protein ID: WP_001372321.1
• Coordinates: 33114..33239 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	32970..33035 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI33_RS23600	27984..28433	-	450	Protein_35	hypothetical protein	-
IGI33_RS22545	28735..29262	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
IGI33_RS22550	29320..29553	+	234	WP_000006003.1	DUF905 family protein	-
IGI33_RS22555	29614..31582	+	1969	Protein_38	ParB/RepB/Spo0J family partition protein	-
IGI33_RS22560	31651..32085	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IGI33_RS22565	32082..32844	+	763	Protein_40	plasmid SOS inhibition protein A	-
-	32813..33037	+	225	NuclAT_0	-	-
-	32813..33037	+	225	NuclAT_0	-	-
-	32813..33037	+	225	NuclAT_0	-	-
-	32813..33037	+	225	NuclAT_0	-	-
IGI33_RS22570	32822..33001	-	180	WP_001309233.1	hypothetical protein	-
-	32970..33035	-	66	-	-	Antitoxin
IGI33_RS23605	33023..33172	+	150	Protein_42	plasmid maintenance protein Mok	-
IGI33_RS22575	33114..33239	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
IGI33_RS22580	33558..33854	-	297	Protein_44	hypothetical protein	-
IGI33_RS22585	34154..34450	+	297	WP_001272251.1	hypothetical protein	-
IGI33_RS22590	34561..35382	+	822	WP_001234445.1	DUF932 domain-containing protein	-
IGI33_RS22595	35679..36269	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
IGI33_RS22600	36604..36987	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
IGI33_RS22605	37181..37852	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
IGI33_RS22610	37989..38216	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T203740 WP_001372321.1 NZ_CP075478:33114-33239 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T203740 NZ_CP100308:1925205-1925307 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 66 bp

>AT203740 NZ_CP075478:c33035-32970 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin