Detailed information of TA system TA203739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4475545..4476147
• Replicon: chromosome
• Accession: NZ_CP075477
• Organism: Escherichia coli strain NT1W25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: IGI33_RS21385
• Protein ID: WP_000897305.1
• Coordinates: 4475836..4476147 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IGI33_RS21380
• Protein ID: WP_000356397.1
• Coordinates: 4475545..4475835 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI33_RS21355 (4471471)	4471471..4472373	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
IGI33_RS21360 (4472370)	4472370..4473005	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
IGI33_RS21365 (4473002)	4473002..4473931	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
IGI33_RS21370 (4474261)	4474261..4474503	-	243	WP_001086388.1	protein YiiF	-
IGI33_RS21375 (4474722)	4474722..4474940	-	219	WP_001295676.1	CopG family transcriptional regulator	-
IGI33_RS21380 (4475545)	4475545..4475835	-	291	WP_000356397.1	NadS family protein	Antitoxin
IGI33_RS21385 (4475836)	4475836..4476147	-	312	WP_000897305.1	hypothetical protein	Toxin
IGI33_RS21390 (4476376)	4476376..4477284	+	909	WP_137446804.1	alpha/beta hydrolase	-
IGI33_RS21395 (4477348)	4477348..4478289	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
IGI33_RS21400 (4478334)	4478334..4478771	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
IGI33_RS21405 (4478768)	4478768..4479640	-	873	WP_000920762.1	virulence factor BrkB family protein	-
IGI33_RS21410 (4479634)	4479634..4480233	-	600	WP_001315111.1	glucose-1-phosphatase	-
IGI33_RS21415 (4480332)	4480332..4481117	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T203739 WP_000897305.1 NZ_CP075477:c4476147-4475836 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T203739 NZ_CP100308:842386-842796 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT203739 WP_000356397.1 NZ_CP075477:c4475835-4475545 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT203739 NZ_CP100308:842139-842405 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7