Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3661246..3661787 Replicon chromosome
Accession NZ_CP075477
Organism Escherichia coli strain NT1W25

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag IGI33_RS17605 Protein ID WP_000615976.1
Coordinates 3661509..3661787 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IGI33_RS17600 Protein ID WP_000729704.1
Coordinates 3661246..3661506 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI33_RS17575 (3657029) 3657029..3657814 - 786 WP_000207590.1 putative lateral flagellar export/assembly protein LafU -
IGI33_RS17580 (3657786) 3657786..3659498 + 1713 Protein_3446 flagellar biosynthesis protein FlhA -
IGI33_RS17585 (3659603) 3659603..3659881 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
IGI33_RS17590 (3659874) 3659874..3660230 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
IGI33_RS17595 (3660287) 3660287..3661060 - 774 WP_000543895.1 C40 family peptidase -
IGI33_RS17600 (3661246) 3661246..3661506 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IGI33_RS17605 (3661509) 3661509..3661787 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IGI33_RS17610 (3661943) 3661943..3662683 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
IGI33_RS17615 (3662654) 3662654..3663421 - 768 WP_000333380.1 class II glutamine amidotransferase -
IGI33_RS17620 (3663627) 3663627..3664205 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3659874..3671399 11525


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T203736 WP_000615976.1 NZ_CP075477:3661509-3661787 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T203736 NZ_CP100308:324717-325085 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT203736 WP_000729704.1 NZ_CP075477:3661246-3661506 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT203736 NZ_CP100308:324499-324720 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References