Detailed information of TA system TA203717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 595201..596000
• Replicon: chromosome
• Accession: NZ_CP075477
• Organism: Escherichia coli strain NT1W25

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: IGI33_RS02970
• Protein ID: WP_000347267.1
• Coordinates: 595201..595665 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: IGI33_RS02975
• Protein ID: WP_001307405.1
• Coordinates: 595665..596000 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI33_RS02940 (590202)	590202..590636	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
IGI33_RS02945 (590654)	590654..591532	-	879	WP_001298758.1	PTS N-acetylgalactosamine transporter subunit IID	-
IGI33_RS02950 (591522)	591522..592301	-	780	WP_166488258.1	PTS N-acetylgalactosamine transporter subunit IIC	-
IGI33_RS02955 (592312)	592312..592785	-	474	WP_001336162.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IGI33_RS02960 (592808)	592808..594088	-	1281	WP_000681947.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IGI33_RS02965 (594337)	594337..595146	+	810	WP_000072180.1	aga operon transcriptional regulator AgaR	-
IGI33_RS02970 (595201)	595201..595665	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IGI33_RS02975 (595665)	595665..596000	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IGI33_RS02980 (596149)	596149..597720	-	1572	WP_001273741.1	galactarate dehydratase	-
IGI33_RS02985 (598095)	598095..599429	+	1335	Protein_575	galactarate/glucarate/glycerate transporter GarP	-
IGI33_RS02990 (599445)	599445..600215	+	771	WP_166488256.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	595201..608267	13066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T203717 WP_000347267.1 NZ_CP075477:c595665-595201 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T203717 NZ_CP100294:c112229-111828 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT203717 WP_001307405.1 NZ_CP075477:c596000-595665 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT203717 NZ_CP100294:c112465-112229 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTCTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7