Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3644294..3644835 Replicon chromosome
Accession NZ_CP075472
Organism Escherichia coli strain NT1F25

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag IGI24_RS18670 Protein ID WP_000615979.1
Coordinates 3644557..3644835 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IGI24_RS18665 Protein ID WP_000729704.1
Coordinates 3644294..3644554 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI24_RS18645 (3639977) 3639977..3640762 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
IGI24_RS18650 (3640734) 3640734..3642446 + 1713 Protein_3554 flagellar biosynthesis protein FlhA -
IGI24_RS18655 (3642662) 3642662..3643159 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
IGI24_RS18660 (3643335) 3643335..3644108 - 774 WP_001353765.1 C40 family peptidase -
IGI24_RS18665 (3644294) 3644294..3644554 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IGI24_RS18670 (3644557) 3644557..3644835 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IGI24_RS18675 (3644991) 3644991..3645731 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
IGI24_RS18680 (3645702) 3645702..3646469 - 768 WP_000333379.1 class II glutamine amidotransferase -
IGI24_RS18685 (3646675) 3646675..3647253 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3593282..3669455 76173


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T203688 WP_000615979.1 NZ_CP075472:3644557-3644835 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T203688 NZ_CP100264:c184763-184413 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT203688 WP_000729704.1 NZ_CP075472:3644294-3644554 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT203688 NZ_CP100264:c185008-184757 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References