Detailed information of TA system TA203687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3638105..3638799
• Replicon: chromosome
• Accession: NZ_CP075472
• Organism: Escherichia coli strain NT1F25

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IGI24_RS18630
• Protein ID: WP_001263493.1
• Coordinates: 3638105..3638503 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IGI24_RS18635
• Protein ID: WP_000554757.1
• Coordinates: 3638506..3638799 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3633765)	3633765..3633845	-	81	NuclAT_11	-	-
- (3633765)	3633765..3633845	-	81	NuclAT_11	-	-
- (3633765)	3633765..3633845	-	81	NuclAT_11	-	-
- (3633765)	3633765..3633845	-	81	NuclAT_11	-	-
IGI24_RS18600 (3633105)	3633105..3634349	-	1245	WP_000189541.1	esterase FrsA	-
IGI24_RS18605 (3634441)	3634441..3634899	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IGI24_RS18610 (3635160)	3635160..3636617	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IGI24_RS18615 (3636674)	3636674..3637195	-	522	Protein_3547	peptide chain release factor H	-
IGI24_RS18620 (3637194)	3637194..3637397	-	204	Protein_3548	RtcB family protein	-
IGI24_RS18625 (3637643)	3637643..3638095	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IGI24_RS18630 (3638105)	3638105..3638503	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IGI24_RS18635 (3638506)	3638506..3638799	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IGI24_RS18640 (3638851)	3638851..3639906	-	1056	WP_193125220.1	DNA polymerase IV	-
IGI24_RS18645 (3639977)	3639977..3640762	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
IGI24_RS18650 (3640734)	3640734..3642446	+	1713	Protein_3554	flagellar biosynthesis protein FlhA	-
IGI24_RS18655 (3642662)	3642662..3643159	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3593282..3669455	76173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T203687 WP_001263493.1 NZ_CP075472:c3638503-3638105 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T203687 NZ_CP100262:2277960-2278142 [Burkholderia glumae]
ATGAACAGCGCGAACCTTATCCGAACCCTGAAAGCCGACGGCTGGTATCTCGTCAACACCGTCGGCTCGCATCACCAGTT
CAAGCATGCGACCAAGCCCGGTAAGGTGACGGTGCCTCACCCGAAGAAAGACCTTCCCCTCGGCACCGTGCGCAGCATCC
TGAAACAAGCCGGTCTGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT203687 WP_000554757.1 NZ_CP075472:c3638799-3638506 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT203687 NZ_CP100262:2278169-2278564 [Burkholderia glumae]
ATGCTCTATCCGCTCTATGTCCACGTCGGTGACGACAAGCACGCCCACGGTGTCACCTTCCCCGACTTTCCCGGCTGCCA
CGCAGCGGCCGACTCCTGGGACGAGCTGCCTGCAGCCGTGCAGGAGGCCGCGCAGGCTCATTTCGAGGGCGACGACAGCC
CGATTCCCGAGCCTTCCGCGCTCGAAGATTTGACGCGCGATCCGGAATACACCGGCGGCGTCTGGATGATCTTCGATATC
GACCTGTCGAAGATCAACACGAAAGCCGTGCGCTTCAACGTCAGCATGTCCGAGCGGCTGCTCCAGAAGATCGACGCCAA
GGCGCGCGCGCGCAAACTCTCGCGATCGGCCTTCCTTGCGATTGCGGCAGAACACGAAATGGAAGCTGACGCCTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6