Detailed information of TA system TA203670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 715489..716182
• Replicon: chromosome
• Accession: NZ_CP075472
• Organism: Escherichia coli strain NT1F25

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: IGI24_RS04135
• Protein ID: WP_000415584.1
• Coordinates: 715489..715785 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: IGI24_RS04140
• Protein ID: WP_000650107.1
• Coordinates: 715787..716182 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI24_RS04105 (710934)	710934..711631	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
IGI24_RS04110 (711821)	711821..712154	+	334	Protein_682	DUF2645 family protein	-
IGI24_RS04115 (712200)	712200..713549	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
IGI24_RS04120 (713546)	713546..714205	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
IGI24_RS04125 (714357)	714357..714749	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
IGI24_RS04130 (714802)	714802..715284	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
IGI24_RS04135 (715489)	715489..715785	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
IGI24_RS04140 (715787)	715787..716182	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
IGI24_RS04145 (716315)	716315..717922	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
IGI24_RS04150 (718060)	718060..720318	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	701674..720318	18644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T203670 WP_000415584.1 NZ_CP075472:715489-715785 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T203670 NZ_CP100245:c1664251-1663754 [Burkholderia glumae]
GTGACGGGCTGGCGCGCGCCCGAACCGCTCAGCGACGCGCACGGGCTCGACGATTTCGATTCCGGCGTCGCGAGCCTCGA
CACGTGGCTCAAACGCCGTGCACTGGCCAATCAGCGTAGCGGTGCGTCGCGGACCTTTGTGGCGACGCGAGACGGTCGAG
TGGGGGCGTACTACGCACTCGCGTCCGGCGCGGTTACGCTCGAGGTCGCACCGGGCCGCTTCCGGCGCAACATGCCCGAT
CCGATACCGGTGGTTGTGCTCGGGCGTCTCGCCGTCGATCGCGCCCATCAGGGCATTGGGGTTGGGCGTGCGCTGGTACG
CGATGCCGGCTTGCGTGTGTTGCACGCCGCAGCGGCCATCGGGATTCGCGGCCTGATCGTGCATGCACTCTCGGACTCGG
CCAAGGCGTTCTATGAGCGGGTCGGCTTTGAGGCGTCGCCGCTCGATCCGATGCTGCTCCTGATCACGCTGGCCGACCTC
GAGCACGCCTTGTCATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT203670 WP_000650107.1 NZ_CP075472:715787-716182 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT203670 NZ_CP100245:c1664529-1664248 [Burkholderia glumae]
ATGACCTCGACCACCCCACGCGACACTCTGAACCTACGCATTCGTCCTGACGATCGCAGCTTGATTGACCGCGCCGCAGC
GGCCGCCCACAAAACGCGTACCGACTTCGTTCTGGACGCAGCGCGGGCCGCCGCCGAACAGACCTTGCTCGACCAGGTTG
TTCTGAGCGCCGAGCCGCAGGCCTACGCGGCCTTCCTGGCGCGTTTGGACGAGCCCGTCCAACCGAACGAGGCATTGCGC
CAGACCATGCAGACACGCGCGCCCTGGGACCCCGCTGCGTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65