Detailed information of TA system TA020367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3973487..3974214
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ECs_3965
• Protein ID: NP_311992.1
• Coordinates: 3973900..3974214 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECs_3964
• Protein ID: NP_311991.1
• Coordinates: 3973487..3973903 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_3962	3968547..3970898	+	2352	NP_311989.1	alpha-glucosidase	-
ECs_3963	3971424..3973442	+	2019	NP_311990.1	2,4-dienoyl-CoA reductase	-
ECs_3964	3973487..3973903	-	417	NP_311991.1	antitoxin of the HigB-HigA toxin-antitoxin system	Antitoxin
ECs_3965	3973900..3974214	-	315	NP_311992.1	mRNA interferase toxin of the HigB-HigA toxin-antitoxin system	Toxin
ECs_3966	3974498..3975634	-	1137	NP_311993.2	23S rRNA m(2)G1835 methyltransferase	-
ECs_3967	3975719..3976222	+	504	NP_311994.2	metal dependent hydrolase	-
ECs_3968	3976299..3976991	+	693	NP_311995.1	hypothetical protein	-
ECs_3969	3977070..3978056	+	987	NP_311996.2	NAD(P)-dependent dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Antitoxin HigA (1-138)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12103.10 Da
• Isoelectric Point: 10.0409

>T20367 NP_311992.1 NC_002695:c3974214-3973900 [Escherichia coli O157:H7 str. Sakai]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T20367 NC_002695:c3974214-3973900 [Escherichia coli O157:H7 str. Sakai]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15016.48 Da
• Isoelectric Point: 4.5805

>AT20367 NP_311991.1 NC_002695:c3973903-3973487 [Escherichia coli O157:H7 str. Sakai]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT20367 NC_002695:c3973903-3973487 [Escherichia coli O157:H7 str. Sakai]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA