Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 650138..650865 Replicon chromosome
Accession NZ_CP075472
Organism Escherichia coli strain NT1F25

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IGI24_RS03825 Protein ID WP_000550189.1
Coordinates 650138..650452 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IGI24_RS03830 Protein ID WP_000560266.1
Coordinates 650449..650865 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI24_RS03805 (646296) 646296..647282 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
IGI24_RS03810 (647361) 647361..648053 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
IGI24_RS03815 (648130) 648130..648633 - 504 WP_001300832.1 M48 family metallopeptidase -
IGI24_RS03820 (648718) 648718..649854 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IGI24_RS03825 (650138) 650138..650452 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IGI24_RS03830 (650449) 650449..650865 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IGI24_RS03835 (650910) 650910..652928 - 2019 WP_000121453.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IGI24_RS03840 (653354) 653354..655705 - 2352 WP_000695495.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T203669 WP_000550189.1 NZ_CP075472:650138-650452 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T203669 NZ_CP100244:2971952-2972134 [Burkholderia glumae]
ATGAACAGCGCGAACCTTATCCGAACCCTGAAAGCCGACGGCTGGTATCTCGTCAATACCGTCGGCTCGCATCACCAGTT
CAAGCATGCGACCAAGCCCGGTAAGGTGACGGTGCCTCACCCGAAGAAAGACCTTCCCCTAGGCACCGTGCGCAGCATCC
TGAAACAAGCCGGTCTGAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT203669 WP_000560266.1 NZ_CP075472:650449-650865 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT203669 NZ_CP100244:2972161-2972556 [Burkholderia glumae]
ATGCTCTATCCGCTCTATGTCCACGTCGGCGACGACAAGCACGCACATGGCGTCACTTTTCCCGACTTCCCCGGCTGCCA
CGCTGCGGCCGACTCCTGGGACGAACTGCCCGCAGCCGTGCAGGAGGCCGCCCAGGCCCATTTCGAGGGCGACGACAGCC
CGATTCCCGAGCCTTCCGCGCTCGAAGATTTGACGCGCGATCCGGAATACACCGGCGGCGTCTGGATGATCTTCGATATC
GACCTGTCGAAGATCAACACGAAAGCCGTGCGCTTCAACGTCAGCATGTCCGAGCGGCTGCTCCAGAAGATCGACGCCAA
GGCGCGCGCGCGCAAACTCTCGCGATCGGCCTTCCTTGCGATTGCGGCAGAACACGAAATGGAAGCTGACGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References