Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 606028..606827 Replicon chromosome
Accession NZ_CP075472
Organism Escherichia coli strain NT1F25

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag IGI24_RS03590 Protein ID WP_000347273.1
Coordinates 606028..606492 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IGI24_RS03595 Protein ID WP_001307405.1
Coordinates 606492..606827 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI24_RS03560 (601029) 601029..601463 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
IGI24_RS03565 (601481) 601481..602359 - 879 WP_193125162.1 PTS N-acetylgalactosamine transporter subunit IID -
IGI24_RS03570 (602349) 602349..603128 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
IGI24_RS03575 (603139) 603139..603612 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IGI24_RS03580 (603635) 603635..604915 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
IGI24_RS03585 (605164) 605164..605973 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IGI24_RS03590 (606028) 606028..606492 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IGI24_RS03595 (606492) 606492..606827 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IGI24_RS03600 (606976) 606976..608547 - 1572 WP_001723936.1 galactarate dehydratase -
IGI24_RS03605 (608922) 608922..610256 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
IGI24_RS03610 (610272) 610272..611042 + 771 WP_193125163.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T203668 WP_000347273.1 NZ_CP075472:c606492-606028 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T203668 NZ_CP100243:c82404-82054 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT203668 WP_001307405.1 NZ_CP075472:c606827-606492 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT203668 NZ_CP100243:c82649-82398 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References