Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3708847..3709388 Replicon chromosome
Accession NZ_CP075469
Organism Escherichia coli strain NT1W22

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag IGI32_RS18160 Protein ID WP_000615983.1
Coordinates 3709110..3709388 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag IGI32_RS18155 Protein ID WP_000729703.1
Coordinates 3708847..3709107 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI32_RS18135 (3704522) 3704522..3705307 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
IGI32_RS18140 (3705279) 3705279..3706991 + 1713 Protein_3583 flagellar biosynthesis protein FlhA -
IGI32_RS18145 (3707215) 3707215..3707712 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IGI32_RS18150 (3707888) 3707888..3708637 - 750 WP_024192679.1 C40 family peptidase -
IGI32_RS18155 (3708847) 3708847..3709107 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IGI32_RS18160 (3709110) 3709110..3709388 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IGI32_RS18165 (3709544) 3709544..3710284 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
IGI32_RS18170 (3710255) 3710255..3711022 - 768 WP_000333380.1 class II glutamine amidotransferase -
IGI32_RS18175 (3711228) 3711228..3711806 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T203663 WP_000615983.1 NZ_CP075469:3709110-3709388 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T203663 NZ_CP100235:10784-11134 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT203663 WP_000729703.1 NZ_CP075469:3708847-3709107 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT203663 NZ_CP100235:10539-10790 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References