Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3702650..3703344 | Replicon | chromosome |
| Accession | NZ_CP075469 | ||
| Organism | Escherichia coli strain NT1W22 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | IGI32_RS18120 | Protein ID | WP_001263493.1 |
| Coordinates | 3702650..3703048 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | IGI32_RS18125 | Protein ID | WP_000554757.1 |
| Coordinates | 3703051..3703344 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IGI32_RS18090 (3698209) | 3698209..3698667 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| IGI32_RS18095 (3698928) | 3698928..3700385 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| IGI32_RS18100 (3700442) | 3700442..3700963 | - | 522 | Protein_3575 | peptide chain release factor H | - |
| IGI32_RS18105 (3700962) | 3700962..3701165 | - | 204 | Protein_3576 | RtcB family protein | - |
| IGI32_RS18110 (3701411) | 3701411..3701863 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| IGI32_RS18115 (3701937) | 3701937..3702634 | + | 698 | WP_223367350.1 | IS1 family transposase | - |
| IGI32_RS18120 (3702650) | 3702650..3703048 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| IGI32_RS18125 (3703051) | 3703051..3703344 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| IGI32_RS18130 (3703396) | 3703396..3704451 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| IGI32_RS18135 (3704522) | 3704522..3705307 | - | 786 | WP_000207587.1 | putative lateral flagellar export/assembly protein LafU | - |
| IGI32_RS18140 (3705279) | 3705279..3706991 | + | 1713 | Protein_3583 | flagellar biosynthesis protein FlhA | - |
| IGI32_RS18145 (3707215) | 3707215..3707712 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3616085..3708514 | 92429 | |
| - | flank | IS/Tn | - | - | 3702341..3702634 | 293 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T203662 WP_001263493.1 NZ_CP075469:c3703048-3702650 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T203662 NZ_CP100235:5464-5997 [Burkholderia glumae]
ATGATCCTCACGCCGCTGCAGGACATTACCGCCTACCGCATGCACGTGCCCAAGTGGGCCGTGGCACCCACCAGTGGCGC
GGGTGCCGCCCGGGCCGGCGGCCGGGCGAACCGGGTTGGCACGCCGGCGCTGTATCTGGCGCTCGAGGTGGCCACCGCAG
TGGCCGAGTACCAGCAGCTGTCGCCGCTGCTGCCGCCCGGCACGCTGGTGAGCTATCGGGTCACGGCGACGCCCGTGGTC
GATTTCACCGGCGGCTTTCGCGCGGATCTCTGGCCGCCTTTGTGGGAATCCTTCTTCTGCGACTGGCGCCGCGACTGGTT
TAATGCGCGCATCGAGCCGCCCAGCTGGGTGCTGGGCGATGAGGCGATCGCCGCCGGCGCAAAAGGGATCCTGTTTCGGT
CGACGCAGGCCACCGGCGGCACGAACCTGGTGCTGTTCGTCGACCAGCTGGGGGAGGGCGATCGCCTGGATGTCCACGAC
CCAGCCGGCGCCTTGCCGCGTGATCAGGCTTCATGGGACGGCCCCTCGAGGTGA
ATGATCCTCACGCCGCTGCAGGACATTACCGCCTACCGCATGCACGTGCCCAAGTGGGCCGTGGCACCCACCAGTGGCGC
GGGTGCCGCCCGGGCCGGCGGCCGGGCGAACCGGGTTGGCACGCCGGCGCTGTATCTGGCGCTCGAGGTGGCCACCGCAG
TGGCCGAGTACCAGCAGCTGTCGCCGCTGCTGCCGCCCGGCACGCTGGTGAGCTATCGGGTCACGGCGACGCCCGTGGTC
GATTTCACCGGCGGCTTTCGCGCGGATCTCTGGCCGCCTTTGTGGGAATCCTTCTTCTGCGACTGGCGCCGCGACTGGTT
TAATGCGCGCATCGAGCCGCCCAGCTGGGTGCTGGGCGATGAGGCGATCGCCGCCGGCGCAAAAGGGATCCTGTTTCGGT
CGACGCAGGCCACCGGCGGCACGAACCTGGTGCTGTTCGTCGACCAGCTGGGGGAGGGCGATCGCCTGGATGTCCACGAC
CCAGCCGGCGCCTTGCCGCGTGATCAGGCTTCATGGGACGGCCCCTCGAGGTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT203662 WP_000554757.1 NZ_CP075469:c3703344-3703051 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT203662 NZ_CP100235:5042-5467 [Burkholderia glumae]
ATGAGCACCCTCGCAGCCACCCGAGCACCCGCCGCGCCAGCCGGCGGCCCGGCCCCGGCAAGCTTTGATGCATTCCTGAG
TGCGTTGCGCGAGCCGGACACGGCAGCCCCGGTGCTGTCGGCCCGTCGCTACGTGGCGGCCCTCAACATCGACCTGCAGA
CGCTCGCCGACCAGGCTCACGTCCACCGCAACACCGTCGCCCGCGCGCCGCAGTCGGCCAGCGTCCAGCAGTTCCTGCGC
GAGGCGATCCGCGTGATCCGCGCCGCGACGGATCTGTCGGGCGACGTCCAGCGCGCGCTGTTCTGGTATCGCAACGAGCC
GCTGGCCGTGTTTGACTACCAGACGGCCGAAACCCTGGTGTCGGCCGGCCGCGCCGATGACGTGGTGCGCTATGTGGCGT
CGCTCGAGGCGGGCGCCGCCGGATGA
ATGAGCACCCTCGCAGCCACCCGAGCACCCGCCGCGCCAGCCGGCGGCCCGGCCCCGGCAAGCTTTGATGCATTCCTGAG
TGCGTTGCGCGAGCCGGACACGGCAGCCCCGGTGCTGTCGGCCCGTCGCTACGTGGCGGCCCTCAACATCGACCTGCAGA
CGCTCGCCGACCAGGCTCACGTCCACCGCAACACCGTCGCCCGCGCGCCGCAGTCGGCCAGCGTCCAGCAGTTCCTGCGC
GAGGCGATCCGCGTGATCCGCGCCGCGACGGATCTGTCGGGCGACGTCCAGCGCGCGCTGTTCTGGTATCGCAACGAGCC
GCTGGCCGTGTTTGACTACCAGACGGCCGAAACCCTGGTGTCGGCCGGCCGCGCCGATGACGTGGTGCGCTATGTGGCGT
CGCTCGAGGCGGGCGCCGCCGGATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|