Detailed information of TA system TA203642

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31714..31983
• Replicon: plasmid pNT1N34-93k
• Accession: NZ_CP075466
• Organism: Escherichia coli strain NT1N34

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: IGI31_RS22135
• Protein ID: WP_001372321.1
• Coordinates: 31858..31983 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	31714..31779 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI31_RS22105 (27424)	27424..27951	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
IGI31_RS22110 (28009)	28009..28242	+	234	WP_000006003.1	DUF905 family protein	-
IGI31_RS22115 (28303)	28303..30326	+	2024	Protein_34	ParB/RepB/Spo0J family partition protein	-
IGI31_RS22120 (30395)	30395..30829	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
IGI31_RS22125 (30826)	30826..31588	+	763	Protein_36	plasmid SOS inhibition protein A	-
IGI31_RS22130 (31566)	31566..31745	-	180	WP_001309233.1	hypothetical protein	-
- (31714)	31714..31779	+	66	NuclAT_1	-	-
- (31714)	31714..31779	-	66	NuclAT_0	-	Antitoxin
- (31714)	31714..31779	-	66	NuclAT_0	-	Antitoxin
- (31557)	31557..31781	+	225	NuclAT_0	-	-
- (31557)	31557..31781	+	225	NuclAT_0	-	-
- (31557)	31557..31781	+	225	NuclAT_0	-	-
- (31557)	31557..31781	+	225	NuclAT_0	-	-
- (31557)	31557..31781	-	225	NuclAT_0	-	-
IGI31_RS23205 (31767)	31767..31916	+	150	Protein_38	plasmid maintenance protein Mok	-
IGI31_RS22135 (31858)	31858..31983	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
IGI31_RS22140 (32302)	32302..32598	-	297	Protein_40	hypothetical protein	-
IGI31_RS22145 (32898)	32898..33194	+	297	WP_001272251.1	hypothetical protein	-
IGI31_RS22150 (33305)	33305..34126	+	822	WP_001234445.1	DUF932 domain-containing protein	-
IGI31_RS22155 (34423)	34423..35025	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
IGI31_RS22160 (35348)	35348..35731	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
IGI31_RS22165 (35925)	35925..36596	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
IGI31_RS22170 (36733)	36733..36960	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T203642 WP_001372321.1 NZ_CP075466:31858-31983 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T203642 NZ_CP100211:c125215-124865 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT203642 NZ_CP075466:c31779-31714 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin