Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3607875..3608416 Replicon chromosome
Accession NZ_CP075465
Organism Escherichia coli strain NT1N34

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag IGI31_RS17395 Protein ID WP_000615976.1
Coordinates 3608138..3608416 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IGI31_RS17390 Protein ID WP_000729704.1
Coordinates 3607875..3608135 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI31_RS17370 (3603550) 3603550..3604335 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
IGI31_RS17375 (3604307) 3604307..3606019 + 1713 Protein_3418 flagellar biosynthesis protein FlhA -
IGI31_RS17380 (3606243) 3606243..3606740 - 498 WP_193107710.1 REP-associated tyrosine transposase RayT -
IGI31_RS17385 (3606916) 3606916..3607689 - 774 WP_015675129.1 C40 family peptidase -
IGI31_RS17390 (3607875) 3607875..3608135 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IGI31_RS17395 (3608138) 3608138..3608416 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IGI31_RS17400 (3608572) 3608572..3609312 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
IGI31_RS17405 (3609283) 3609283..3610050 - 768 WP_000333380.1 class II glutamine amidotransferase -
IGI31_RS17410 (3610256) 3610256..3610834 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / IlpA 3580568..3648835 68267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T203640 WP_000615976.1 NZ_CP075465:3608138-3608416 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T203640 NZ_CP100208:c123326-122925 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT203640 WP_000729704.1 NZ_CP075465:3607875-3608135 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT203640 NZ_CP100208:c123562-123326 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References