Detailed information of TA system TA203619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 597512..598311
• Replicon: chromosome
• Accession: NZ_CP075465
• Organism: Escherichia coli strain NT1N34

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: IGI31_RS02895
• Protein ID: WP_000347273.1
• Coordinates: 597512..597976 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: IGI31_RS02900
• Protein ID: WP_021547164.1
• Coordinates: 597976..598311 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI31_RS02865 (592513)	592513..592947	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
IGI31_RS02870 (592965)	592965..593843	-	879	WP_001300474.1	PTS N-acetylgalactosamine transporter subunit IID	-
IGI31_RS02875 (593833)	593833..594612	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
IGI31_RS02880 (594623)	594623..595096	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IGI31_RS02885 (595119)	595119..596399	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IGI31_RS02890 (596648)	596648..597457	+	810	WP_130061339.1	aga operon transcriptional regulator AgaR	-
IGI31_RS02895 (597512)	597512..597976	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IGI31_RS02900 (597976)	597976..598311	-	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IGI31_RS02905 (598460)	598460..600031	-	1572	WP_001753084.1	galactarate dehydratase	-
IGI31_RS02910 (600406)	600406..601740	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
IGI31_RS02915 (601756)	601756..602526	+	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T203619 WP_000347273.1 NZ_CP075465:c597976-597512 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T203619 NZ_CP100184:c158270-157860 [Burkholderia glumae]
ATGACGCTGTACCTGCTCGACACCAACATCTTGTCCAGCGTGATTCGAGACCCGCGCGGCGCCTGCGCGACCCGGATCGG
ACACACTCAGCCCGAGCAAATTTGCACCTCGATCATCGTCGCGGCGGAACTGCGGTTTGGCGTCTTCAAGCGCGGCTCGC
CGACCCTCGCGCAGCGCGTCGAGCAACTGCTCGCGAGCTTGAGCGTGCTGCCGTTGCAGCCCGATGCTGACCAGTGCTAT
GGCCGGTTGCGGGCCGACCTTGAGAAGCAGGGCCAGTTGATCGGCGCCAACGACATGCTGATCGCCGCGCACGCGCTGGC
GCTCGAGGCCGTCCTGGTGACGGACAACACGGCGGAATTTGCCCGTGTCGCGGGGCTTCCGGTCGAAAACTGGTTGCGGC
CGACGCCGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT203619 WP_021547164.1 NZ_CP075465:c598311-597976 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT203619 NZ_CP100184:c158509-158267 [Burkholderia glumae]
ATGCCCGCACATGACACCCGCCATGCCCGATTGTTTCGCAACGGGCGCAGTCAGGCCGTGCGGATTCCGCGCGAGTTCGA
GCTTTCCGCGTCCGAGGTCACGATCTATCGCGAGGGCGCACGTTTGATCATCGAGCCCGTCCAGGCCGCGCCCTTGCGTG
AGCTGTTCGCCCAATGGACCCCGCTGGACGAGACCTTCCCCGACATCGAGAACCTGCCGGCCGAACCGGTCGACATCGAA
TGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39