Detailed information of TA system TA203617

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64681..64950
• Replicon: plasmid pNT1F10-102k
• Accession: NZ_CP075464
• Organism: Escherichia coli strain NT1F10

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: IGI19_RS24225
• Protein ID: WP_001372321.1
• Coordinates: 64825..64950 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	64681..64746 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI19_RS24640	59789..60064	+	276	WP_074156329.1	hypothetical protein	-
IGI19_RS24645	60305..60511	+	207	WP_000547968.1	hypothetical protein	-
IGI19_RS24195	60537..60989	+	453	WP_097331204.1	single-stranded DNA-binding protein	-
IGI19_RS24200	61051..61284	+	234	WP_000006030.1	DUF905 family protein	-
IGI19_RS24205	61349..63307	+	1959	WP_096309213.1	ParB/RepB/Spo0J family partition protein	-
IGI19_RS24210	63362..63796	+	435	WP_096309212.1	conjugation system SOS inhibitor PsiB	-
IGI19_RS24215	63793..64555	+	763	Protein_68	plasmid SOS inhibition protein A	-
IGI19_RS24220	64524..64712	-	189	WP_001299721.1	hypothetical protein	-
-	64524..64748	+	225	NuclAT_0	-	-
-	64524..64748	+	225	NuclAT_0	-	-
-	64524..64748	+	225	NuclAT_0	-	-
-	64524..64748	+	225	NuclAT_0	-	-
-	64681..64746	-	66	-	-	Antitoxin
IGI19_RS24650	64734..64883	+	150	Protein_70	plasmid maintenance protein Mok	-
IGI19_RS24225	64825..64950	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
IGI19_RS24655	65170..65400	+	231	WP_072279680.1	hypothetical protein	-
IGI19_RS24660	65398..65571	-	174	Protein_73	hypothetical protein	-
IGI19_RS24665	65641..65847	+	207	WP_000547968.1	hypothetical protein	-
IGI19_RS24230	65872..66159	+	288	WP_000107548.1	hypothetical protein	-
IGI19_RS24235	66278..67099	+	822	WP_001234469.1	DUF932 domain-containing protein	-
IGI19_RS24240	67396..67998	-	603	WP_112918141.1	transglycosylase SLT domain-containing protein	-
IGI19_RS24245	68319..68702	+	384	WP_166498013.1	conjugal transfer relaxosome DNA-binding protein TraM	-
IGI19_RS24250	68889..69578	+	690	WP_137440653.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat	1..102015	102015

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T203617 WP_001372321.1 NZ_CP075464:64825-64950 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T203617 NZ_CP100183:11502-11852 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT203617 NZ_CP075464:c64746-64681 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin