Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3721500..3722041 Replicon chromosome
Accession NZ_CP075462
Organism Escherichia coli strain NT1F10

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag IGI19_RS18730 Protein ID WP_000615983.1
Coordinates 3721763..3722041 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag IGI19_RS18725 Protein ID WP_000729703.1
Coordinates 3721500..3721760 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI19_RS18705 (3717175) 3717175..3717960 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
IGI19_RS18710 (3717932) 3717932..3719644 + 1713 Protein_3599 flagellar biosynthesis protein FlhA -
IGI19_RS18715 (3719868) 3719868..3720365 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IGI19_RS18720 (3720541) 3720541..3721290 - 750 WP_024192679.1 C40 family peptidase -
IGI19_RS18725 (3721500) 3721500..3721760 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IGI19_RS18730 (3721763) 3721763..3722041 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IGI19_RS18735 (3722197) 3722197..3722937 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
IGI19_RS18740 (3722908) 3722908..3723675 - 768 WP_000333380.1 class II glutamine amidotransferase -
IGI19_RS18745 (3723881) 3723881..3724459 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T203613 WP_000615983.1 NZ_CP075462:3721763-3722041 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T203613 NZ_CP100179:c125420-125019 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT203613 WP_000729703.1 NZ_CP075462:3721500-3721760 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT203613 NZ_CP100179:c125656-125420 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References