Detailed information of TA system TA203612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3715303..3715997
• Replicon: chromosome
• Accession: NZ_CP075462
• Organism: Escherichia coli strain NT1F10

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IGI19_RS18690
• Protein ID: WP_001263493.1
• Coordinates: 3715303..3715701 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IGI19_RS18695
• Protein ID: WP_000554757.1
• Coordinates: 3715704..3715997 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGI19_RS18660 (3710862)	3710862..3711320	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IGI19_RS18665 (3711581)	3711581..3713038	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IGI19_RS18670 (3713095)	3713095..3713616	-	522	Protein_3591	peptide chain release factor H	-
IGI19_RS18675 (3713615)	3713615..3713818	-	204	Protein_3592	RtcB family protein	-
IGI19_RS18680 (3714064)	3714064..3714516	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IGI19_RS18685 (3714590)	3714590..3715287	+	698	WP_223367350.1	IS1 family transposase	-
IGI19_RS18690 (3715303)	3715303..3715701	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IGI19_RS18695 (3715704)	3715704..3715997	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IGI19_RS18700 (3716049)	3716049..3717104	-	1056	WP_001226164.1	DNA polymerase IV	-
IGI19_RS18705 (3717175)	3717175..3717960	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
IGI19_RS18710 (3717932)	3717932..3719644	+	1713	Protein_3599	flagellar biosynthesis protein FlhA	-
IGI19_RS18715 (3719868)	3719868..3720365	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3714994..3715287	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T203612 WP_001263493.1 NZ_CP075462:c3715701-3715303 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T203612 NZ_CP100178:c92333-92031 [Burkholderia glumae]
ATGCGGGGTAAGGCGCGGGTGGTGCGGCTGACGCCCTTGGCCGAGGCCGACCTCGAGGACATCTGGGCTTACATCTTCGA
GCAGTGGTCACTCGCGCAGGCCGAGCAATACGTCGGCGAGATCGTCGCTGCCTTCAACCGACTGGCGCGCGGCGAATGGG
TCGGGGCCGTGCGGGGCGAACCTTACCGGCGCTATCTGGTCGGCTTCCATGCGGTGTTTTATCGAGAGACCACCGACGCC
TTGGATGTCGCCTGTGTGCGGCATCAGCGTATGGACGTGGGTTCGCACCTGTCGCAGTATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT203612 WP_000554757.1 NZ_CP075462:c3715997-3715704 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT203612 NZ_CP100178:c92568-92323 [Burkholderia glumae]
ATGCCGCGGAACACATCGATCTCGCTGGGGGATCACCTGACTCAGTTTGTCGACACACAGGTCGCATCCGGCCGCTATGG
CTCGGCCAGCGACGTGGTGCGCGCCGGGCTTCGCCTGCTCGAGACGCAGGAAACCGAACTGAAGGCGCTGAAGGAGGCGT
TGCGCGCCAGTGAGGCGTCCGGCGCGCCCGCGGCGTTTGACGGCCAAGCGTTCCTCGACCGCATGCGTGCGAAGCATGCG
GGGTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6