Detailed information of TA system TA203581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3888041..3888638
• Replicon: chromosome
• Accession: NZ_CP075452
• Organism: Klebsiella pneumoniae strain RGF20-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: KI738_RS19100
• Protein ID: WP_004893639.1
• Coordinates: 3888321..3888638 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KI738_RS19095
• Protein ID: WP_004142561.1
• Coordinates: 3888041..3888328 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI738_RS19065 (3884248)	3884248..3884496	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
KI738_RS19070 (3884514)	3884514..3884855	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KI738_RS19075 (3884886)	3884886..3886001	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
KI738_RS19080 (3886181)	3886181..3886762	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
KI738_RS19085 (3886762)	3886762..3887130	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
KI738_RS19090 (3887250)	3887250..3887903	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
KI738_RS19095 (3888041)	3888041..3888328	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KI738_RS19100 (3888321)	3888321..3888638	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI738_RS19105 (3888823)	3888823..3889866	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
KI738_RS19110 (3890536)	3890536..3891402	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
KI738_RS19115 (3891511)	3891511..3892938	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T203581 WP_004893639.1 NZ_CP075452:c3888638-3888321 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T203581 NZ_CP100142:5823-6356 [Burkholderia glumae]
ATGATCCTCACGCCGTTGCAGGACATCACGGCCTATCGCATGCATGTGCCCAAGTGGGCCGTCGCGCCCACCAGCGGCGC
TGGCGCGGCCAAGGCCGGGGGGCGGGCCAACCGGATCGGTACGCCAGCGTTGTACCTGGCGCTGGAGGCGGCCACCGCGG
TGGCCGAGTACCAGCAGCTGTCGCCCCTGTTGCCGCCGGGCACGTTGGTGAGCTATCGCGTCACGGCCACGCCCGTGGTC
GATTTCACCGGCGGCTACCGTGTGGATCTCTGGCCGCCCCTCTGGGAGTCGTTCTTCTGCGACTGGCGGCATGACTGGTT
CAACGCGCGCATTGAGCCGCCCAGCTGGGTACTCGGCGACGAGGCAATCGCCTCCGGCGCCAAGGGCATCCTGTTTCGCT
CGACGCAGGCCGCCGGCGGCACGAACTTGGTGCTGTTCGTCGACCAGTTGGGGGAGGGCGATCGCGTGGATGTTCACGAC
CCGGCCGGCGCCTTGCCGCGCGATCAAGCGTCGTGGGTTGACTCGCCCGAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT203581 WP_004142561.1 NZ_CP075452:c3888328-3888041 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT203581 NZ_CP100142:5410-5826 [Burkholderia glumae]
ATGAGCACTCTCGCAGCAGCGCGAGCCCCCGCCGGCGGTCCGGCCCTGGCAAACTTTGACGCGTTTCTCAGCGCGCTACG
CGAGCCGGACACGGCCGCTCCCGTGCTGTCGGCTCGTCGCTACGTGGCGGCCCTCAACATCGACCTGCAGACGCTCGCGG
ACCAGGCTCACGTCCATCGCAACACCGTCGCGCGCGCGCCGCAGTCAGCCAGCGTCCAGCAGTTTCTGCGCGAGGCGATT
CGCGTGATCCGAGCCGCGACGGATCTGTCGGGCGACGTTCAACGCGCGCTGTTCTGGTATCGCAACGAGCCTCTGGCCGT
CTTCGATTACCAGACGGCCGAAACGCTGGTGTCGGCAGGGCGCGCCGATGACGTTGTGCGCTATGTGGCATCGCTCGAGG
CGGGCGCCGCCGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1