Detailed information of TA system TA203562

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31076..31340
• Replicon: plasmid unnamed4
• Accession: NZ_CP075377
• Organism: Escherichia coli strain no107

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: KIB02_RS24965
• Protein ID: WP_001331364.1
• Coordinates: 31188..31340 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	31076..31133 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIB02_RS24950 (27118)	27118..28188	-	1071	WP_213833234.1	DsbC family protein	-
KIB02_RS24955 (28207)	28207..29415	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
KIB02_RS24960 (29633)	29633..30586	-	954	WP_021513958.1	hypothetical protein	-
- (30780)	30780..30835	-	56	NuclAT_1	-	-
- (30780)	30780..30835	-	56	NuclAT_1	-	-
- (30780)	30780..30835	-	56	NuclAT_1	-	-
- (30780)	30780..30835	-	56	NuclAT_1	-	-
- (31076)	31076..31133	-	58	NuclAT_0	-	Antitoxin
- (31076)	31076..31133	-	58	NuclAT_0	-	Antitoxin
- (31076)	31076..31133	-	58	NuclAT_0	-	Antitoxin
- (31076)	31076..31133	-	58	NuclAT_0	-	Antitoxin
KIB02_RS24965 (31188)	31188..31340	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
KIB02_RS24970 (31412)	31412..31663	-	252	WP_001291964.1	hypothetical protein	-
KIB02_RS27090 (32162)	32162..32257	+	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
KIB02_RS24975 (32322)	32322..32498	-	177	WP_001054901.1	hypothetical protein	-
KIB02_RS24980 (32830)	32830..33039	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
KIB02_RS24985 (33111)	33111..33773	-	663	WP_060527638.1	plasmid IncI1-type surface exclusion protein ExcA	-
KIB02_RS24990 (33844)	33844..36012	-	2169	WP_000698368.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..89891	89891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T203562 WP_001331364.1 NZ_CP075377:31188-31340 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T203562 NZ_CP100121:91884-92300 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT203562 NZ_CP075377:c31133-31076 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin