Detailed information of TA system TA203557

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31068..31332
• Replicon: plasmid unnamed3
• Accession: NZ_CP075376
• Organism: Escherichia coli strain no107

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: KIB02_RS24465
• Protein ID: WP_001331364.1
• Coordinates: 31180..31332 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	31068..31125 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIB02_RS24450 (27110)	27110..28180	-	1071	WP_213833234.1	DsbC family protein	-
KIB02_RS24455 (28199)	28199..29407	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
KIB02_RS24460 (29625)	29625..30578	-	954	WP_021513958.1	hypothetical protein	-
- (30772)	30772..30827	-	56	NuclAT_1	-	-
- (30772)	30772..30827	-	56	NuclAT_1	-	-
- (30772)	30772..30827	-	56	NuclAT_1	-	-
- (30772)	30772..30827	-	56	NuclAT_1	-	-
- (31068)	31068..31125	-	58	NuclAT_0	-	Antitoxin
- (31068)	31068..31125	-	58	NuclAT_0	-	Antitoxin
- (31068)	31068..31125	-	58	NuclAT_0	-	Antitoxin
- (31068)	31068..31125	-	58	NuclAT_0	-	Antitoxin
KIB02_RS24465 (31180)	31180..31332	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
KIB02_RS24470 (31404)	31404..31655	-	252	WP_001291964.1	hypothetical protein	-
KIB02_RS27085 (32154)	32154..32249	+	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
KIB02_RS24475 (32314)	32314..32490	-	177	WP_001054901.1	hypothetical protein	-
KIB02_RS24480 (32822)	32822..33031	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
KIB02_RS24485 (33103)	33103..33765	-	663	WP_060527638.1	plasmid IncI1-type surface exclusion protein ExcA	-
KIB02_RS24490 (33836)	33836..36004	-	2169	WP_000698368.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88195	88195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T203557 WP_001331364.1 NZ_CP075376:31180-31332 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T203557 NZ_CP100120:c4774195-4773662 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT203557 NZ_CP075376:c31125-31068 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin