Detailed information of TA system TA203555

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31389..31658
• Replicon: plasmid unnamed2
• Accession: NZ_CP075375
• Organism: Escherichia coli strain no107

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KIB02_RS24065
• Protein ID: WP_001372321.1
• Coordinates: 31533..31658 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	31389..31454 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIB02_RS24035	27099..27626	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
KIB02_RS24040	27684..27917	+	234	WP_000006003.1	DUF905 family protein	-
KIB02_RS24045	27978..30001	+	2024	Protein_40	ParB/RepB/Spo0J family partition protein	-
KIB02_RS24050	30070..30504	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KIB02_RS24055	30501..31263	+	763	Protein_42	plasmid SOS inhibition protein A	-
-	31232..31456	+	225	NuclAT_0	-	-
-	31232..31456	+	225	NuclAT_0	-	-
-	31232..31456	+	225	NuclAT_0	-	-
-	31232..31456	+	225	NuclAT_0	-	-
KIB02_RS24060	31241..31420	-	180	WP_001309233.1	hypothetical protein	-
-	31389..31454	-	66	-	-	Antitoxin
KIB02_RS26935	31442..31591	+	150	Protein_44	plasmid maintenance protein Mok	-
KIB02_RS24065	31533..31658	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KIB02_RS24070	31977..32273	-	297	Protein_46	hypothetical protein	-
KIB02_RS24075	32572..32868	+	297	WP_001272251.1	hypothetical protein	-
KIB02_RS24080	32979..33800	+	822	WP_001234445.1	DUF932 domain-containing protein	-
KIB02_RS24085	34097..34699	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
KIB02_RS24090	35020..35403	+	384	WP_053320747.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KIB02_RS24095	35590..36279	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T203555 WP_001372321.1 NZ_CP075375:31533-31658 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T203555 NZ_CP100120:c3970104-3969787 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT203555 NZ_CP075375:c31454-31389 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin