Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 644480..645279 Replicon chromosome
Accession NZ_CP075374
Organism Escherichia coli strain no107

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag KIB02_RS03140 Protein ID WP_000347266.1
Coordinates 644480..644944 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KIB02_RS03145 Protein ID WP_001307405.1
Coordinates 644944..645279 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIB02_RS03110 (639481) 639481..639915 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KIB02_RS03115 (639933) 639933..640811 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
KIB02_RS03120 (640801) 640801..641580 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KIB02_RS03125 (641591) 641591..642064 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KIB02_RS03130 (642087) 642087..643367 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
KIB02_RS03135 (643616) 643616..644425 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KIB02_RS03140 (644480) 644480..644944 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KIB02_RS03145 (644944) 644944..645279 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KIB02_RS03150 (645428) 645428..646999 - 1572 WP_001273741.1 galactarate dehydratase -
KIB02_RS03155 (647374) 647374..648708 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KIB02_RS03160 (648724) 648724..649494 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T203537 WP_000347266.1 NZ_CP075374:c644944-644480 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T203537 NZ_CP100114:4060106-4060324 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT203537 WP_001307405.1 NZ_CP075374:c645279-644944 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT203537 NZ_CP100114:4059705-4060079 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References