Detailed information of TA system TA203532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4836516..4837270
• Replicon: chromosome
• Accession: NZ_CP075372
• Organism: Salmonella enterica strain no75

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: KH432_RS23805
• Protein ID: WP_000558166.1
• Coordinates: 4836516..4836827 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KH432_RS23810
• Protein ID: WP_001259011.1
• Coordinates: 4836824..4837270 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH432_RS23775 (4832174)	4832174..4833076	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
KH432_RS23780 (4833073)	4833073..4833708	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
KH432_RS23785 (4833705)	4833705..4834634	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
KH432_RS23790 (4834681)	4834681..4834971	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
KH432_RS23795 (4834972)	4834972..4835283	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
KH432_RS23800 (4835501)	4835501..4836430	+	930	WP_001127703.1	alpha/beta hydrolase	-
KH432_RS23805 (4836516)	4836516..4836827	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
KH432_RS23810 (4836824)	4836824..4837270	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
KH432_RS23815 (4837285)	4837285..4838226	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
KH432_RS23820 (4838271)	4838271..4838708	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
KH432_RS23825 (4838705)	4838705..4839577	-	873	WP_000921427.1	virulence factor BrkB family protein	-
KH432_RS23830 (4839571)	4839571..4840170	-	600	WP_000965695.1	glucose-1-phosphatase	-
KH432_RS23835 (4840361)	4840361..4841164	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
KH432_RS23840 (4841198)	4841198..4842094	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T203532 WP_000558166.1 NZ_CP075372:4836516-4836827 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T203532 NZ_CP100114:346296-346664 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT203532 WP_001259011.1 NZ_CP075372:4836824-4837270 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT203532 NZ_CP100114:346078-346299 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90