Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3527500..3528149 Replicon chromosome
Accession NZ_CP075345
Organism Enterobacter kobei strain S18_CRE18

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0PLQ3
Locus tag KJE18_RS16870 Protein ID WP_014882864.1
Coordinates 3527500..3527862 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2W0GCS7
Locus tag KJE18_RS16875 Protein ID WP_014882863.1
Coordinates 3527850..3528149 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJE18_RS16850 (KJE18_16820) 3522837..3523808 - 972 WP_014882868.1 LacI family DNA-binding transcriptional regulator -
KJE18_RS16855 (KJE18_16825) 3523884..3525026 - 1143 WP_023336852.1 Gfo/Idh/MocA family oxidoreductase -
KJE18_RS16860 (KJE18_16830) 3525019..3526044 - 1026 WP_270094221.1 sugar phosphate isomerase/epimerase family protein -
KJE18_RS16865 (KJE18_16835) 3526060..3527294 - 1235 Protein_3299 MFS transporter -
KJE18_RS16870 (KJE18_16840) 3527500..3527862 + 363 WP_014882864.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KJE18_RS16875 (KJE18_16845) 3527850..3528149 + 300 WP_014882863.1 helix-turn-helix transcriptional regulator Antitoxin
KJE18_RS16880 (KJE18_16850) 3528260..3529648 - 1389 WP_014882862.1 phenylalanine transporter -
KJE18_RS16885 (KJE18_16855) 3529751..3531724 - 1974 WP_023336850.1 PhoX family phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-80)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13952.92 Da        Isoelectric Point: 6.7114

>T203501 WP_014882864.1 NZ_CP075345:3527500-3527862 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGNKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Download         Length: 363 bp

>T203501 NZ_CP100106:698418-698801 [Klebsiella pneumoniae]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10860.61 Da        Isoelectric Point: 9.8627

>AT203501 WP_014882863.1 NZ_CP075345:3527850-3528149 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Download         Length: 300 bp

>AT203501 NZ_CP100106:697960-698328 [Klebsiella pneumoniae]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0PLQ3

Antitoxin

Source ID Structure
AlphaFold DB A0A2W0GCS7

References