Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 289892..290586 Replicon chromosome
Accession NC_002695
Organism Escherichia coli O157:H7 str. Sakai

Toxin (Protein)


Gene name yafO Uniprot ID -
Locus tag ECs_0260 Protein ID NP_308287.1
Coordinates 290188..290586 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID -
Locus tag ECs_0259 Protein ID NP_308286.1
Coordinates 289892..290185 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECs_0256 286245..287984 - 1740 NP_308284.2 flagellar export pore protein -
ECs_0257 288079..288714 + 636 NP_308283.2 flagellar system protein MbhA -
ECs_0258 288785..289840 + 1056 NP_308285.1 DNA polymerase IV -
ECs_0259 289892..290185 + 294 NP_308286.1 antitoxin of the YafO-YafN toxin-antitoxin system Antitoxin
ECs_0260 290188..290586 + 399 NP_308287.1 mRNA interferase toxin of the YafO-YafN toxin-antitoxin system Toxin
ECs_0261 290671..291048 + 378 NP_308288.2 acetyltransferase -
ECs_0262 291355..291621 + 267 NP_308289.1 hypothetical protein -
ECs_0263 291590..292090 + 501 NP_308290.1 peptide chain release factor RF-1 -
ECs_0264 292147..293604 - 1458 NP_308291.1 aminoacyl-histidine dipeptidase -
ECs_0265 293865..294323 + 459 NP_308292.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T20349 NP_308287.1 NC_002695:290188-290586 [Escherichia coli O157:H7 str. Sakai]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T20349 NC_002695:290188-290586 [Escherichia coli O157:H7 str. Sakai]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT20349 NP_308286.1 NC_002695:289892-290185 [Escherichia coli O157:H7 str. Sakai]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT20349 NC_002695:289892-290185 [Escherichia coli O157:H7 str. Sakai]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References